Diosmectite-zinc oxide composite improves intestinal barrier restoration and modulates TGF-β1, ERK1/2, and Akt in piglets after acetic acid challenge

被引:26
作者
Song, Z. -H. [1 ]
Ke, Y. -L. [1 ]
Xiao, K. [1 ]
Jiao, L. -F. [1 ]
Hong, Q. -H. [1 ]
Hu, C. -H. [1 ]
机构
[1] Zhejiang Univ, Anim Sci Coll, Key Lab Mol Anim Nutr, Minist Educ, Hangzhou 310058, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Akt; diosmectite-zinc oxide composite; ERK1/2; piglets; restoration; TGF-beta; 1; ACTIVATED PROTEIN-KINASES; GROWTH-FACTOR-I; WEANED PIGS; SIGNALING PATHWAYS; WEANLING PIGS; EPITHELIAL-CELLS; BONE-GROWTH; EXPRESSION; BETA; INFLAMMATION;
D O I
10.2527/jas.2014-8580
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The present study evaluated the beneficial effect of diosmectite-zinc oxide composite (DS-ZnO) on improving intestinal barrier restoration in piglets after acetic acid challenge and explored the underlying mechanisms. Twenty-four 35-d-old piglets (Duroc x Landrace x Yorkshire), with an average weight of 8.1 kg, were allocated to 4 treatment groups. On d 1 of the trial, colitis was induced via intrarectal injection of acetic acid (10 mL of 10% acetic acid [ACA] solution for ACA, DS-ZnO, and mixture of diosmectite [DS] and ZnO [DS+ZnO] groups) and the control group was infused with saline. Twenty-four hours after challenged, piglets were fed with the following diets: 1) control group (basal diet), 2) ACA group (basal diet), 3) DS-ZnO group (basal diet supplemented with DS-ZnO), and 4) DS+ZnO group (mixture of 1.5 g diosmectite [DS]/kg and 500 mg Zn/kg from ZnO [equal amount of DS and ZnO in the DS-ZnO treatment group]). On d 8 of the trial, piglets were sacrificed. The results showed that DS-ZnO supplementation improved (P < 0.05) ADG, ADFI, and transepithelial electrical resistance and decreased (P < 0.05) fecal scores, crypt depth, and fluorescein isothiocyanate-dextran 4kDa (FD4) influx as compared with ACA group. Moreover, DS-ZnO increased (P < 0.05) occludin, claudin-1, and zonula occluden-1 expressions; reduced (P < 0.05) caspase-9 and caspase-3 activity and Bax expression; and improved (P < 0.05) Bcl2, XIAP, and PCNA expression. Diosmectite-zinc oxide composite supplementation also increased (P < 0.05) TGF-beta 1 expression and ERK1/2 and Akt activation. These results suggest that DS-ZnO attenuates the acetic acid-induced colitis by improving mucosa barrier restoration, inhibiting apoptosis, and improving intestinal epithelial cells proliferation and modulation of TGF-beta 1 and ERK1/2 and Akt signaling pathway.
引用
收藏
页码:1599 / 1607
页数:9
相关论文
共 40 条
[1]   Shikonin promotes intestinal wound healing in vitro via induction of TGF-β release in IEC-18 cells [J].
Andujar, I. ;
Rios, J. L. ;
Giner, R. M. ;
Recio, M. C. .
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2013, 49 (04) :637-641
[2]  
[Anonymous], 2012, TABL FEED COMP NUTR
[3]  
[Anonymous], 1998, NUTR REQ SWIN
[4]   Restoration of barrier function in injured intestinal mucosa [J].
Blikslager, Anthony T. ;
Moeser, Adam J. ;
Gookin, Jody L. ;
Jones, Samuel L. ;
Odle, Jack .
PHYSIOLOGICAL REVIEWS, 2007, 87 (02) :545-564
[5]   Weaning induces both transient and long-lasting modifications of absorptive, secretory, and barrier properties of piglet intestine [J].
Boudry, G ;
Péron, V ;
Le Huërou-Luron, I ;
Lallès, JP ;
Sève, B .
JOURNAL OF NUTRITION, 2004, 134 (09) :2256-2262
[6]   HB-EGF enhances restitution after intestinal ischemia/reperfusion via PI3K/Akt and MEK/ERK1/2 activation [J].
El-Assal, ON ;
Besner, GE .
GASTROENTEROLOGY, 2005, 129 (02) :609-625
[7]   Zinc Protects Human Kidney Cells from Depleted Uranium-induced Apoptosis [J].
Hao, Yuhui ;
Ren, Jiong ;
Liu, Cong ;
Li, Hong ;
Liu, Jing ;
Yang, Zhangyou ;
Li, Rong ;
Su, Yongping .
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY, 2014, 114 (03) :271-280
[8]  
Hollis GR, 2005, J ANIM SCI, V83, P2123
[9]   Dietary supplementation with tributyrin alleviates intestinal injury in piglets challenged with intrarectal administration of acetic acid [J].
Hou, Yongqing ;
Wang, Lei ;
Yi, Dan ;
Ding, Binying ;
Chen, Xing ;
Wang, Qingjing ;
Zhu, Huiling ;
Liu, Yulan ;
Yin, Yulong ;
Gong, Joshua ;
Wu, Guoyao .
BRITISH JOURNAL OF NUTRITION, 2014, 111 (10) :1748-1758
[10]   Effects of zinc oxide supported on zeolite on growth performance, intestinal microflora and permeability, and cytokines expression of weaned pigs [J].
Hu, C. H. ;
Xiao, K. ;
Song, J. ;
Luan, Z. S. .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 2013, 181 (1-4) :65-71