Characterization and application of a novel laccase derived from Bacillus amyloliquefaciens

被引：41

作者：

Wang, Hongbin ^{[1
]}

Huang, Lin ^{[1
]}

Li, Yanzhen ^{[1
]}

Ma, Jieying ^{[1
]}

Wang, Shuang ^{[1
]}

Zhang, Yuanfu ^{[1
]}

Ge, Xiuqi ^{[1
]}

Wang, Nan ^{[1
]}

Lu, Fuping ^{[1
]}

Liu, Yihan ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Tianjin Key Lab Ind Microbiol, Natl Engn Lab Ind Enzymes,Coll Biotechnol,Minist, Tianjin 300457, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 150卷

基金：

中国国家自然科学基金; 中国博士后科学基金; 国家重点研发计划;

关键词：

Laccase; Bacillus amyloliquefaciens; Expression; Characterization; Decolorization; ALKALI-STABLE LACCASE; PUMILUS COTA-LACCASE; BIOCHEMICAL-CHARACTERIZATION; EXTRACELLULAR LACCASE; BACTERIAL LACCASE; CRYSTAL-STRUCTURE; PICHIA-PASTORIS; BLUE LACCASE; EXPRESSION; SUBTILIS;

D O I：

10.1016/j.ijbiomac.2019.11.117

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As the copper-containing enzymes, laccases demonstrate a promising potential in various environmental and industrial applications. In this study, a bacterial strain isolated from soil exhibited the laccase activity, which was subsequently characterized and named as Bacillus amyloliquefaciens TCCC 111018. The novel gene encoding CotA-laccase (lac) was amplified using the genome of B. amyloliquefaciens TCCC 111018 as the template and efficiently and actively expressed in Escherichia coli. The recombinant LAC (rLAC) exhibited its highest activity at 80 degrees C and pH 5.5 for 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) oxidization and 80 degrees C and pH 7.0 for 2,6-dimethoxyphenol (2,6-DMP) oxidization. rLAC was stable at up to 60 degrees C and within the pH ranging from 3.0 to 9.0 when using the substrate ABTS. Furthermore, rLAC demonstrated the relatively high tolerance to NaCI, SDS, and most metal ions. Moreover, rIAC was capable of decolorizing the structurally different azo, anthraquinone, and triphenylmethane with different mediator at 60 degrees C under pH 5.5, 7.0, and 9.0. Therefore, rLAC would be an ideal candidate for lots of biotechnological and industrial applications due to its stability in the extreme conditions, including but not limit to pH, high temperature, halides, heavy metals and detergents. (C) 2019 Elsevier B.V. All tights reserved.

引用

页码：982 / 990

页数：9

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