The treatment outcome of synovial sarcoma is poor owing to its resistance to radiation and chemotherapy. The Transducin-like Enhancer of Split 1 (TLE1) is a co-repressor that involves many signaling pathways like cell survival, hematopoiesis and differentiation. Although TLE1 is uniquely expressed in synovial sarcomas, the biological role of TLE1 is not completely understood. This study evaluated the function of TLE1 in synovial sarcomas using knock-down of TLE1, and examined whether the inhibition of TLE1 suppresses the proliferation of synovial sarcomas and enhances the cytotoxicity caused by doxorubicin (doxo). The over-expression of TLE1 was first confirmed in synovial sarcoma cells (HS-SYII). When the HS-SYII cells and normal fibroblast were transiently transfected with TLE1 siRNA, the MTT assay revealed growth inhibition in the HS-SY-11 cells but not in the normal fibroblast. TLE1 silencing also potentiated the cytotoxic effects of doxo against HS-SYII cells. This effect of TLE1 silencing was attributed mainly to the induction of apoptosis. Subsequent analysis revealed that Bcl-2 is a possible downstream target of TLE1 signaling. This study demonstrated that TLE1 is a critical factor for the survival of synovial sarcomas. Overall, the inhibition of TLE1 affects cell proliferation and the apoptosis pathway by suppressing the expression of Bcl-2. (C) 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29: 1131-1136, 2011
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Sichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China
He, Xin
Xiong, Bo
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Sichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China
Xiong, Bo
Zhou, Tingqing
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Mianyang Peoples Hosp, Dept Pathol, Mianyang 621000, Sichuan, Peoples R ChinaSichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China
Zhou, Tingqing
Lan, Ting
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Sichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China
Lan, Ting
Chen, Min
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Sichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China
Chen, Min
Chen, Huijiao
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Sichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China
Chen, Huijiao
Peng, Ran
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Sichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China
Peng, Ran
Guo, Jia
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Sichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China
Guo, Jia
Zhang, Hongying
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Sichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, West China Hosp, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China
Zhang, Hongying
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,
2016,
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