Optimization of the coupling of high-temperature liquid chromatography and flame ionization detection application to the separations of alcohols

被引：46

作者：

Guillarme, D ^{[1
]}

Heinisch, S ^{[1
]}

Gauvrit, JY ^{[1
]}

Lanteri, P ^{[1
]}

Rocca, JL ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS, UMR 5180, Analyt Sci Lab, F-69622 Villeurbanne, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1078卷 / 1-2期

关键词：

high temperature liquid chromatography (HTLC); HTLC-FID coupling; separation of alcohols;

D O I：

10.1016/j.chroma.2005.04.092

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The feasibility of coupling high-temperature liquid chromatography (HTLC) to flame ionization detection (FID) has been studied. FID parameter values (hydrogen flow-rate, air flow-rate and FID temperature), typically set in gas chromatography are rarely suitable for liquid chromatography. Best values depend obviously on the water flow rate which is defined depending on both column temperature and column internal diameter. The FID parameters were optimized according to the water flow-rate by means of an experimental design. The potential of the method is shown with some alcohol separations and the value of increasing column temperature while reducing the column diameter is highlighted. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：22 / 27

页数：6

共 20 条

[1]

[Anonymous], 1984, PERRYS CHEM ENG HDB

[2]

ANTIA FD, 1988, J CHROMATOGR, V435, P1

[3] Simultaneous flame ionization and absorbance detection of volatile and nonvolatile compounds by reversed-phase liquid chromatography with a water mobile phase [J].

Bruckner, CA ;

Ecker, ST ;

Synovec, RE .

ANALYTICAL CHEMISTRY, 1997, 69 (17) :3465-3470

[4] Reversed phase liquid chromatography of organic hydrocarbons with water as the mobile phase [J].

Foster, MD ;

Synovec, RE .

ANALYTICAL CHEMISTRY, 1996, 68 (17) :2838-2844

[5] Effect of temperature in reversed phase liquid chromatography [J].

Guillarme, D ;

Heinisch, S ;

Rocca, JL .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1052 (1-2) :39-51

[6] EXTRACTION OF ORGANIC POLLUTANTS FROM ENVIRONMENTAL SOLIDS WITH SUBCRITICAL AND SUPERCRITICAL WATER [J].

HAWTHORNE, SB ;

YANG, Y ;

MILLER, DJ .

ANALYTICAL CHEMISTRY, 1994, 66 (18) :2912-2920

[7]

Hooijschuur EWJ, 2000, HRC-J HIGH RES CHROM, V23, P309

[8] Separation of nucleosides and their bases by reversed-phase liquid chromatography using pure water as the mobile phase [J].

Hu, WZ ;

Hasebe, K ;

Reynolds, DM ;

Haraguchi, H .

ANALYTICA CHIMICA ACTA, 1997, 353 (01) :143-149

[9]

Ingelse BA, 1998, HRC-J HIGH RES CHROM, V21, P613

[10] Effect of temperature on the thermodynamic properties, kinetic performance, and stability of polybutadiene-coated zirconia [J].

Li, JW ;

Carr, PW .

ANALYTICAL CHEMISTRY, 1997, 69 (05) :837-843

← 1 2 →