Effect of beta-glucan in lung damage secondary to experimental obstructive jaundice

被引:5
作者
Erkol, Hayri [1 ]
Kahramansoy, Nurettin [1 ]
Kordon, Ozgur [1 ]
Buyukasik, Oktay [1 ]
Serin, Erdinc [2 ]
Kukner, Aysel [3 ]
机构
[1] Abant Izzet Baysal Univ, Fac Med, Dept Gen Surg, Bolu, Turkey
[2] Abant Izzet Baysal Univ, Fac Med, Dept Biochem, Bolu, Turkey
[3] Abant Izzet Baysal Univ, Fac Med, Dept Histol & Embryol, Bolu, Turkey
关键词
Obstructive jaundice; beta-glucan; lung damage; oxidative damage; antioxidants; OXIDATIVE ORGAN INJURY; EXPERIMENTAL BILIARY OBSTRUCTION; LIPID-PEROXIDATION; RAT MODEL; SACCHAROMYCES-CEREVISIAE; BACTERIAL TRANSLOCATION; STRESS; PROTECTS; MELATONIN; KIDNEY;
D O I
10.4318/tjg.2012.0396
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background/aims: This study aimed at investigating the protective effects of beta-glucans on the lungs in obstructive jaundice. Methods: In total, five groups -Sham (1), control (2) and treatment groups (3,4,5)- were established; each comprising randomly selected seven Wistar Albino rats. Beta-glucan was given after choleduct ligation in Group 3 while it was given before and after the choleduct ligation in Group 4. As pre-treatment beta-glucan was given before ligation in Group 5. Beta-glucan was administered in a single dose of 50 mg / kg / day by gavage for a ten-day period. Superoxide dismutase, and myeloperoxidase levels in serum; malotzdialdehyde, lipid hydroxyperoxidase and glutathione levels in lung tissue; lactate dehydrogenase levels in bronchoalveolar lavage fluid were measured. Results: The blood polymorphonuclear leukocytes level was highest in the control group and lower in the sham and treatment groups. Serum superoxide dismutase and tissue glutathione values were significantly higher in Groups 3 and 4 (p <= 0.04) whilst Groups 3 and 4 did not differ from each other. In Groups 3 and 4 malondialdehyde, lipid hydroxyperoxidase, and myeloperoxidase values were significantly lower. However, Groups 3 and 4 did not differ for malondialdehyde or lipid hydroxyperoxidase values. Lactate dehydrogenase level in the bronchoalveolar lavage fluid was significantly lower in all of the treatment groups (Groups 3,4,5) (p <= 0.008). When compared to the control group, it was observed that lung damage was much more limited in the treatment groups (p<0.001). Conclusion: This study suggests that beta-glucan exhibits protective effect in pulmonary tissue against oxidative damage in obstructive jaundice.
引用
收藏
页码:38 / 45
页数:8
相关论文
共 36 条
[21]   Reactive oxygen species and vascular cell adhesion molecule-1 in distant organ failure following bile duct obstruction in mice [J].
Morise, Z ;
Ueda, M ;
Kitajima, M ;
Epstein, CJ ;
Granger, DN ;
Grisham, MB .
DIGESTIVE DISEASES AND SCIENCES, 2002, 47 (03) :607-613
[22]   Bile duct ligation and oxidative stress in the rat: effects in liver and kidney [J].
Orellana, M ;
Rodrigo, R ;
Thielemann, L ;
Guajardo, V .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 2000, 126 (02) :105-111
[23]   Endotoxinemia in the portal and the systemic circulation in obstructive jaundice [J].
Papakostas, C ;
Bezirtzoglou, E ;
Pitiakoudis, M ;
Polychronidis, A ;
Simopoulos, C .
CLINICAL AND EXPERIMENTAL MEDICINE, 2003, 3 (02) :124-128
[24]   Immunomodulatory activities associated with β-glucan derived from Saccharomyces cerevisiae [J].
Pelizon, AC ;
Kaneno, R ;
Soares, AMVC ;
Meira, DA ;
Sartori, A .
PHYSIOLOGICAL RESEARCH, 2005, 54 (05) :557-564
[25]   Oxidative stress and regulation of glutathione in lung inflammation [J].
Rahman, I ;
MacNee, W .
EUROPEAN RESPIRATORY JOURNAL, 2000, 16 (03) :534-554
[26]   Oral delivery and gastrointestinal absorption of soluble glucans stimulate increased resistance to infectious challenge [J].
Rice, PJ ;
Adams, EL ;
Ozment-Skelton, T ;
Gonzalez, AJ ;
Goldman, MP ;
Lockhart, BE ;
Barker, LA ;
Breuel, KF ;
DePonti, WK ;
Kalbfleisch, JH ;
Ensley, HE ;
Brown, GD ;
Gordon, S ;
Williams, DL .
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2005, 314 (03) :1079-1086
[27]   Pressure ulcer-induced oxidative organ injury is ameliorated by β-glucan treatment in rats [J].
Sener, G ;
Sert, G ;
Sehirli, AÖ ;
Arbak, S ;
Uslu, B ;
Gedik, N ;
Ayanoglu-Dulger, G .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2006, 6 (05) :724-732
[28]   Protective effect of β-glucan against oxidative organ injury in a rat model of sepsis [J].
Sener, G ;
Toklu, H ;
Ercan, F ;
Erkanli, G .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2005, 5 (09) :1387-1396
[29]   β-Glucan protects against chronic nicotine-induced oxidative damage in rat kidney and bladder [J].
Sener, Goksel ;
Toklu, Hale Z. ;
Cetinel, Sule .
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2007, 23 (01) :25-32
[30]   β-glucan ameliorates methotrexate-induced oxidative organ injury via its antioxidant and immunomodulatory effects [J].
Sener, Goksel ;
Eksioglu-Demiralp, Emel ;
Cetiner, Mustafa ;
Ercan, Feriha ;
Yegen, Berrak C. .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2006, 542 (1-3) :170-178