Developmental methylation of the coding region of c-fos occurs perinatally, stepwise and sequentially in the liver of laboratory mouse

被引：5

作者：

Sachan, Manisha ^{[2
]}

Raman, Rajiva ^{[1
]}

机构：

[1] Banaras Hindu Univ, Dept Zool, Cytogenet Lab, Varanasi 221005, Uttar Pradesh, India

[2] CSJM Univ, Dept Biotechnol, Inst Biosci & Biotechnol, Kanpur 208024, Uttar Pradesh, India

来源：

GENE | 2008年 / 416卷 / 1-2期

关键词：

c-fos; de novo methylation; methyltransferase;

D O I：

10.1016/j.gene.2008.02.024

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have studied the dynamics of de novo DNA methylation of 16 contiguous CpGs in the non-CpG island-coding region of the proto-oncogene c-fos during mouse development by Na-bisulfite sequencing. Methylation commences from 16.5 dpc and occurs in stepwise-manner. In liver 7 sites are methylated between 16.5 dpc and day 5 after birth, but all the sites are completely methylated on 20 dpp and remain so in the adult liver. The present study provides evidence that (1) pattern of methylation of c-fos is distinct from those DNA sequences which methylate pre- and post-implantation, both in terms of the timing and spreading, and (2) spacing of CpGs is an important factor in determining the course of methylation. We suggest that there could be other isoforms of Dnmtases for the c-fos like embryonic genes, not only because they methylate later in development but also because of the difference in kinetics of the reaction, and that the nucleation of certain methylated sites facilitate methylation of neighbouring sites and their maintenance in subsequent cell generations. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：22 / 29

页数：8

共 49 条

[1]

Baylin SB, 1998, ADV CANCER RES, V72, P141

[2] The DNA methyltransferases of mammals [J].

Bestor, TH .

HUMAN MOLECULAR GENETICS, 2000, 9 (16) :2395-2402

[3]

BEVEREN CV, 1983, CELL, V32, P1241

[4] PRIMARY DNA-SEQUENCE DETERMINES SITES OF MAINTENANCE AND DENOVO METHYLATION BY MAMMALIAN DNA METHYLTRANSFERASES [J].

BOLDEN, AH ;

NALIN, CM ;

WARD, CA ;

POONIAN, MS ;

WEISSBACH, A .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (04) :1135-1140

[5] SP1 ELEMENTS PROTECT A CPG ISLAND FROM DE-NOVO METHYLATION [J].

BRANDEIS, M ;

FRANK, D ;

KESHET, I ;

SIEGFRIED, Z ;

MENDELSOHN, M ;

NEMES, A ;

TEMPER, V ;

RAZIN, A ;

CEDAR, H .

NATURE, 1994, 371 (6496) :435-438

[6]

Chandrasekhar K, 1997, MOL REPROD DEV, V48, P421

[7] Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b [J].

Chen, TP ;

Ueda, Y ;

Dodge, JE ;

Wang, ZJ ;

Li, E .

MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (16) :5594-5605

[8]

CLARK SJ, 1994, NUCLEIC ACIDS RES, V22, P2290

[9] The H19 methylation imprint is erased and re-established differentially on the parental alleles during male germ cell development [J].

Davis, TL ;

Yang, GJ ;

McCarrey, JR ;

Bartolomei, MS .

HUMAN MOLECULAR GENETICS, 2000, 9 (19) :2885-2894

[10] Acquisition of the H19 methylation imprint occurs differentially on the parental alleles during spermatogenesis [J].

Davis, TL ;

Trasler, JM ;

Moss, SB ;

Yang, GJ ;

Bartolomei, MS .

GENOMICS, 1999, 58 (01) :18-28

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