Exploring the Interaction of Cobalt Oxide Nanoparticles with Albumin, Leukemia Cancer Cells and Pathogenic Bacterial by Multispectroscopic, Docking, Cellular and Antibacterial Approaches

被引:31
作者
Arsalan, Niloofar [1 ]
Kashi, Elahe Hassan [2 ]
Hasan, Anwarul [3 ,4 ]
Doost, Mona Edalat [5 ]
Rasti, Behnam [6 ]
Paray, Bilal Ahamad [7 ]
Nakhjiri, Mona Zahed [1 ]
Sari, Soyar [2 ]
Shari, Majid [8 ]
Shahpasand, Koorosh [9 ]
Akhtari, Keivan [10 ]
Haghighat, Setareh [5 ]
Falahati, Mojtaba [8 ]
机构
[1] Islamic Azad Univ IAU, Dept Biol, Fac Basic Sci, Lahijan Branch, Lahijan, Guilan, Iran
[2] Islamic Azad Univ, Dept Cellular & Mol Biol, Fac Adv Sci & Technol, Tehran Med Sci, Tehran, Iran
[3] Qatar Univ, Dept Mech & Ind Engn, Coll Engn, Doha 2713, Qatar
[4] Qatar Univ, Biomed Res Ctr, Doha 2713, Qatar
[5] Islamic Azad Univ, Dept Microbiol, Fac Adv Sci & Technol, Tehran Med Sci, Tehran, Iran
[6] Islamic Azad Univ IAU, Dept Microbiol, Fac Basic Sci, Lahijan, Guilan, Iran
[7] King Saud Univ, Dept Zool, Coll Sci, Riyadh 11451, Saudi Arabia
[8] Islamic Azad Univ, Dept Nanotechnol, Fac Adv Sci & Technol, Tehran Med Sci, Tehran, Iran
[9] Royan Inst Stem Cell Biol & Technol RI SCBT, Dept Stem Cells & Dev Biol, Cell Sci Res Ctr, Tehran, Iran
[10] Univ Kurdistan, Dept Phys, Sanandaj, Iran
关键词
cobalt oxide; nanoparticle; synthesis; spectroscopy; docking; anticancer; antibacterial; HUMAN-SERUM-ALBUMIN; IN-VITRO; DRUG-DELIVERY; NANOCOMPOSITES; GENOTOXICITY; ANTICANCER; TOXICITY; GRAPHENE; DIOXIDE; BINDING;
D O I
10.2147/IJN.S257711
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Aim: The interaction of NPs with biological systems may reveal useful details about their pharmacodynamic, anticancer and antibacterial effects. Methods: Herein, the interaction of as-synthesized Co3O4 NPs with HSA was explored by different kinds of fluorescence and CD spectroscopic methods, as well as molecular docking studies. Also, the anticancer effect of Co3O4 NPs against leukemia K562 cells was investigated by MTT, LDH, caspase, real-time PCR, ROS, cell cycle, and apoptosis assays. Afterwards, the antibacterial effects of Co3O4 NPs against three pathogenic bacteria were disclosed by antibacterial assays. Results: Different characterization methods such as TEM, DLS, zeta potential and XRD studies proved that fabricated Co3O4 NPs by sol-gel method have a diameter of around 50 nm, hydrodynamic radius of 177 nm with a charge distribution of -33.04 mV and a well-defined crystalline phase. Intrinsic, extrinsic, and synchronous fluorescence as well as CD studies, respectively, showed that the HSA undergoes some fluorescence quenching, minor conformational changes, microenvironmental changes as well as no structural changes in the secondary structure, after interaction with Co3O4 NPs. Molecular docking results also verified that the spherical clusters with a dimension of 1.5 nm exhibit the most binding energy with HSA molecules. Anticancer assays demonstrated that Co3O4 NPs can selectively lead to the reduction of K562 cell viability through the cell membrane damage, activation of caspase-9, -8 and -3, elevation of Bax/Bc1-2 mRNA ratio, ROS production, cell cycle arrest, and apoptosis. Finally, antibacterial assays disclosed that Co3O4 NPs can stimulate a promising antibacterial effect against pathogenic bacteria. Conclusion: In general, these observations can provide useful information for the early stages of nanomaterial applications in therapeutic platforms.
引用
收藏
页码:4607 / 4623
页数:17
相关论文
共 72 条
[1]   Investigating the Interaction of Fe Nanoparticles with Lysozyme by Biophysical and Molecular Docking Studies [J].
Aghili, Zahra ;
Taheri, Saba ;
Zeinabad, Hojjat Alizadeh ;
Pishkar, Leila ;
Saboury, Ali Akbar ;
Rahimi, Arash ;
Falahati, Mojtaba .
PLOS ONE, 2016, 11 (10)
[2]   Synthesis and antibacterial effects of cobalt-cellulose magnetic nanocomposites [J].
Alahmadi, N. S. ;
Betts, J. W. ;
Cheng, F. ;
Francesconi, M. G. ;
Kelly, S. M. ;
Kornherr, A. ;
Prior, T. J. ;
Wadhawan, J. D. .
RSC ADVANCES, 2017, 7 (32) :20020-20026
[3]   Novel route for the preparation of cobalt oxide nanoparticles/reduced graphene oxide nanocomposites and their antibacterial activities [J].
Alsharaeh, Edreese ;
Mussa, Yasmin ;
Ahmed, Faheem ;
Aldawsari, Yazeed ;
Al-Hindawi, Mohammed ;
Sing, Garwin Kim .
CERAMICS INTERNATIONAL, 2016, 42 (02) :3407-3410
[4]  
Amiri S, 2013, Mater Sci Eng C Mater Biol Appl, V33, P1, DOI 10.1016/j.msec.2012.09.003
[5]   Albumin binding, anticancer and antibacterial properties of synthesized zero valent iron nanoparticles [J].
Anbouhi, Tabassom Sedaghat ;
Esfidvajani, Elnaz Mokhtari ;
Nemati, Fahimeh ;
Haghighat, Setareh ;
Sari, Soyar ;
Attar, Farnoosh ;
Pakaghideh, Arezoo ;
Sohrabi, Mohammad Javad ;
Mousavi, Seyyedeh Elaheh ;
Falahati, Mojtaba .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2019, 14 :243-256
[6]  
[Anonymous], 2017, J HYDROSCIENCES ENV
[7]   Probing the conformational changes and peroxidase-like activity of cytochrome c upon interaction with iron nanoparticles [J].
Azad, Vida Jafari ;
Kasravi, Shahab ;
Zeinabad, Hojjat Alizadeh ;
Aval, Mehri Memar Bashi ;
Saboury, Ali Akbar ;
Rahimi, Arash ;
Falahati, Mojtaba .
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2017, 35 (12) :2565-2577
[8]  
Azizi M, 2017, PLOS ONE, V12, DOI [10.1371/Journal.pone.0188639, 10.1371/journal.pone.0188639]
[9]   Albumin binding and anticancer effect of magnesium oxide nanoparticles [J].
Behzadi, Elham ;
Sarsharzadeh, Rozhin ;
Nouri, Mina ;
Attar, Farnoosh ;
Akhtari, Keivan ;
Shahpasand, Koorosh ;
Falahati, Mojtaba .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2019, 14 :257-270
[10]   Nano silica-carbon-silver ternary hybrid induced antimicrobial composite films for food packaging application [J].
Biswas, Manik C. ;
Tiimob, Boniface J. ;
Abdela, Woubit ;
Jeelani, Shaik ;
Rangari, Vijaya K. .
FOOD PACKAGING AND SHELF LIFE, 2019, 19 :104-113