Genotypic diversity and antifungal susceptibility of Cryptococcus neoformans isolates from paediatric patients in China

被引：13

作者：

Hong, Nan ^{[1
,2
]}

Chen, Min ^{[1
,2
]}

Xu, Nan ^{[3
]}

Al-Hatmi, Abdullah M. S. ^{[4
,5
,6
]}

Zhang, Chao ^{[1
,2
]}

Pan, Wei H. ^{[1
,2
]}

Hagen, Ferry ^{[4
]}

Boekhout, Teun ^{[1
,2
,7
,8
]}

Xu, Jianping ^{[9
]}

Zou, Xian B. ^{[10
]}

Liao, Wan Q. ^{[1
,2
]}

机构：

[1] Second Mil Med Univ, Changzheng Hosp, Dept Dermatol, Shanghai, Peoples R China

[2] Second Mil Med Univ, Changzheng Hosp, Shanghai Key Lab Med Mycol, Shanghai, Peoples R China

[3] Tongji Univ, Sch Med, Shanghai East Hosp, Dept Dermatol, Shanghai, Peoples R China

[4] Westerdijk Fungal Biodivers Inst, Dept Med Mycol, Utrecht, Netherlands

[5] Radboud Univ Nijmegen, Med Ctr, Canisius Wilhelmina Hosp, Ctr Expertise Mycol, Nijmegen, Netherlands

[6] Minist Hlth, Directorate Gen Hlth Serv, Ibri, Oman

[7] Westerdijk Fungal Biodivers Inst, Dept Yeast & Basidiomycete Res, Utrecht, Netherlands

[8] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands

[9] McMaster Univ, Dept Biol, Hamilton, ON, Canada

[10] Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Dept Dermatol, Beijing, Peoples R China

来源：

MYCOSES | 2019年 / 62卷 / 02期

基金：

中国国家自然科学基金;

关键词：

antifungal susceptibility; China; Cryptococcus neoformans; genotype; paediatric; EPIDEMIOLOGIC CUTOFF VALUES; GATTII SPECIES COMPLEX; POPULATION-BASED SURVEILLANCE; END-POINT DISTRIBUTIONS; MENINGITIS; INFECTION; FLUCONAZOLE; PROVINCE; ALPHA;

D O I：

10.1111/myc.12863

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Cryptococcosis is a life-threatening mycosis primarily occurring in adult patients particularly those with immunosuppression such as HIV infection/AIDS. The number of reported cases of paediatric cryptococcosis has increased in the last decade around the world, including China. However, current information on the characteristics of cryptococcosis in children, particularly the genotypic diversity and antifungal susceptibility of the isolates, is limited. In the present study, a total of 25 paediatric isolates of Cryptococcus neoformans were genotyped using the ISHAM-MLST scheme. In vitro susceptibility to antifungal agents of the 22 isolates was tested using the CLSI M27-A3 method. Our analyses revealed that the genotypic diversity of C. neoformans isolates from Chinese paediatric patients was low, with ST 5 (80%) and ST 31 (12%) being the two major sequence types. Reduced susceptibility to fluconazole (FLU), 5-flucytosine (5-FC) and itraconazole (ITR) was observed among C. neoformans isolates from Chinese paediatric patients, particularly among the ST5 isolates, which was similar to observations made on C. neoformans isolates from Chinese adult patients. In addition, the majority of isolates (3/4, 75%) obtained from deceased patients showed decreased antifungal susceptibility, which indicates that further monitoring of antifungal susceptibility of Cryptococcus isolates is warranted in management of paediatric cryptococcosis.

引用

页码：171 / 180

页数：10

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