Comparison of two chemiluminescent immunoassays in the detection of measles IgM antibodies

被引:7
作者
Gomez-Camarasa, Cristina [1 ]
Lara-Oya, Ana [1 ]
Cobo, Fernando [1 ]
Sampedro-Martinez, Antonio [1 ]
Rodriguez-Granger, Javier [1 ]
Gutierrez-Fernandez, Jose [1 ]
Maria Navarro-Mari, Jose [1 ]
机构
[1] Univ Hosp Virgen Nieves, Dept Microbiol, Ave Fuerzas Armadas S-N, E-18014 Granada, Spain
关键词
Measles; Serology; Chemiluminescent immunoassay; ELISA;
D O I
10.1016/j.jviromet.2016.08.018
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Serological confirmation of measles is achieved by detecting the specific immunoglobulin M (IgM), and it is important to evaluate new commercial inmunoassays in order to ensure the quality of results. The objective of this study was to compare the performance of a novel automated chemiluminescent immunoassay (CLIA), Virclia IgM measles (Vircell, Spain), with that of the widely used Liaison measles IgM assay (DiaSorin, Italy). A panel of 86 sera from laboratory-confirmed cases was used for the sensitivity calculation, and 59 sera from healthy individuals and those with other viral infections were used for the specificity calculation. Sensitivity values were 96.5% for Virclia and 97.6% for Liaison; specificity values were 93.2% for Virclia and 96.6% for Liaison; neither difference was statistically significant VirClia IgM measles is a good alternative to other immunoassays for the serological confirmation of measles. (C) 2016 Published by Elsevier B.V.
引用
收藏
页码:38 / 39
页数:2
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