Structural characterization of oligosaccharides in the milk of an African elephant (Loxodonta africana africana)

被引：18
作者：
Osthoff, G. [1 ]
DickenS, L. [1 ]
Urashima, T. [3 ]
Bonnet, S. L. [2 ]
Uemura, Y. [3 ]
van der Westhuizen, J. H. [2 ]
机构：
[1] Univ Orange Free State, Dept Microbial Biochem & Food Biotechnol, ZA-9300 Bloemfontein, South Africa
[2] Univ Orange Free State, Dept Chem, ZA-9300 Bloemfontein, South Africa
[3] Obihiro Univ Agr & Vet Med, Dept Bioresource Chem, Obihiro, Hokkaido 0808555, Japan
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2008年 / 150卷 / 01期
关键词：
African elephant; Loxodonta africana; milk; oligosaccharide; type II chain; NMR spectroscopy; nutrition;
D O I：
10.1016/j.cbpb.2008.01.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The oligosaccharides present in the milk of an African elephant (Loxodonta africana africana), collected 4 days post partum, were separated by size exclusion-, anion exchange- and high-performance liquid chromatography (HPLC) before characterisation by H-1 NMR spectroscopy. Neutral and acidic oligosaccharides were identified. Neutral oligosaccharides characterised were isoglobotriose, Gal(beta 1-4)[Fuc(alpha 1-3)]GlcNAc (beta 1-3)Gal(beta 1-4)Glc, Gal(beta 1-4)[Fuc(alpha 1-3)]GleNAc(beta 1-3)Gal(beta 1-4)[Fuc(alpha 1-3)]GlcNAc(beta 1-3)Gal(beta 1-4)Glc, Gal(alpha 1-3)Gal(beta 1-4)[Fuc (alpha-3)]GlcNAc(beta 1-3)Gal(beta 1-4)Glc and a novel oligosaccharide that has not been reported in the milk or colostrum of any other mammal: Gal(alpha 1-3)Gal(beta 1-4)[Fuc(alpha 1-3)]GlcNAc(beta 1-3)Gal(beta 1-4)[Fuc(alpha 1-3)]GleNAc(beta 1-3)Gal(beta 1-4)Glc. Acidic oligosaccharides that are also found in the milk of Asian elephant were Neu5Ac(alpha 2-3)Gal(beta 1-4)Glc, Neu5Ac(alpha 2-6)Gal(beta 1-4)Glc, Neu5Ac(alpha 2-3)Gal(beta 1-4)[Fuc(alpha 1-3)] Glc, Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-3)Gal(beta 1-4)Glc, Neu5Ac(alpha 2-3)Gal(beta 1-4)[Fuc(alpha 1-3)]GlcNAc(beta 1-3)Gal(beta 1-4)Glc, Neu5Ac(alpha 2-6)Ga](beta 1-4)GlcNAc( beta 1-3)Gal(beta 1-4)[Fuc(alpha 1-3)]GlcNAc(beta 1-3)Gal(beta 1-4)Glc and Neu 5Ac(alpha 2 -6)Gal(beta 1-4)GlcNAc(beta 1-3) {Gal(alpha 1 -3)Gal (beta 1-4)[Fuc(alpha 1-3)]GlcNAc(beta 1-6)}Gal(beta 1-4)Glc, while Neu5Gc(alpha 2-3)Gal(beta 1-4)Glc, Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-3)Gal(beta 1-4) [Fuc(alpha 1-3)]Glc, Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-3)[Gal(beta 1-4)GlcNAc(beta 1-6)]Gal(beta 1-4)Glc and Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc (beta 1-3) {Gal(beta 1-4)[Fuc(alpha 1-3)]GlcNAc(beta 1-6)} Gal(beta 1-4)Glc have not been found in Asian elephant milk. The oligosaccharides characterised contained both alpha(2-3)- and alpha(2-6)-linked Neu5Ac residues. They also contain only the type II chain, as found in most non-human, eutherian mammals. (C) 2008 Elsevier Inc. All rights reserved.
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页码：74 / 84
页数：11
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