Simultaneous detection of airborne Aflatoxin, Ochratoxin and Zearalenone in a poultry house by immunoaffinity clean-up and high-performance liquid chromatography

被引：77

作者：

Wang, Yaling ^{[1
,2
]}

Chai, Tongjie ^{[1
]}

Lu, Guozhong ^{[2
]}

Quan, Chunsan ^{[2
]}

Duan, Huiyong ^{[1
]}

Yao, Meiling ^{[1
]}

Zucker, Bert-Andree ^{[3
]}

Schlenker, Gerd ^{[3
]}

机构：

[1] Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China

[2] Dalian Nationalities Univ, Coll Life Sci, Dalian, Peoples R China

[3] Free Univ Berlin, Inst Anim Hyg & Environm Protect, D-1000 Berlin, Germany

来源：

ENVIRONMENTAL RESEARCH | 2008年 / 107卷 / 02期

基金：

中国国家自然科学基金;

关键词：

poultry house; airborne mycotoxins; simultaneous detection of trace aflatoxins; ochratoxin and zearalenone; immunoaffinity column; high-performance liquid chromatography;

D O I：

10.1016/j.envres.2008.01.008

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

An AOZ method, based on high-performance liquid chromatography (HPLC), was optimized on HPLC condition such as mobile phase and wavelength to simultaneously quantify six kinds of mycotoxins [four aflatoxins (AFs), ochratoxin A (OTA) and zearalenone (ZEA)]. Conditions for immunoaffinity clean-up, HPLC and photo-derivatization were optimized in this study and successfully applied in assessment of airborne mycotoxins from a poultry house in Dalian, China. Fifty-two air samples were collected with AGI-30 air samplers using pure water as collection media. Twenty air samples (20/52, 38.46%) were positive for four toxins. Among the positive samples, airborne mycotoxin c ncentrations (mean +/- S.D.) for AFG2, AFBI, and ZEA were 0.189 +/- 0.024 (n = 9), 0.080 +/- 0.003 (n = 11) and 2.363 +/- 0.030 (n = 5) ng/m(3) air, while the concentration for OTA was 8.530 (n = 1) ng/m(3). No positive sample was found for either AFG(1) or AFB(2). A chicken may inhale 0.019-0.057ng AFG(2), 0.013-0.019ng AFB(1), 0.436-0.513ng ZEA, and 1.706ng OTA, respectively, in a day. A poultry worker may inhale 0.504-1.512 ng AFB(1), 0.752-2.28 ng AFG(2), 68.240 ng OTA, and 17.432-20.512 ng ZEA in a working day. This is the first report on airborne mycotoxins in poultry house. These data may have importance in animal and public health implications. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：139 / 144

页数：6

共 39 条

[1] Mycotoxins [J].

Bennett, JW ;

Klich, M .

CLINICAL MICROBIOLOGY REVIEWS, 2003, 16 (03) :497-+

[2]

BRACHMAN PS, 1964, SCIENCE, V144, P1295

[3] Exposure assessment to ochratoxin A from the consumption of Italian and Hungarian wines [J].

Brera, C ;

Soriano, JM ;

Debegnach, F ;

Miraglia, M .

MICROCHEMICAL JOURNAL, 2005, 79 (1-2) :109-113

[4]

BRUCE BJ, 2005, APPL MICROBIOL BIOT, V66, P367

[5]

BURG WR, 1984, J ASSOC OFF ANA CHEM, V67, P309

[6] Simultaneous determination of aflatoxins and ochratoxin A in food using a fully automated immunoaffinity column clean-up and liquid chromatography-fluorescence detection [J].

Chan, D ;

MacDonald, SJ ;

Boughtflower, V ;

Brereton, P .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1059 (1-2) :13-16

[7]

CHE F, 2004, AIRBORNE BIOL THEORY, P5

[8]

CHEN YP, 2003, J CELLULOSE SCI TECH, V11, P19

[9] ACUTE INHALATION TOXICITY OF T-2 MYCOTOXIN IN THE RAT AND GUINEA-PIG [J].

CREASIA, DA ;

THURMAN, JD ;

WANNEMACHER, RW ;

BUNNER, DL .

FUNDAMENTAL AND APPLIED TOXICOLOGY, 1990, 14 (01) :54-59

[10] Mycotoxins of Aspergillus fumigatus in pure culture and in native bioaerosols from compost facilities. [J].

Fischer, G ;

Müller, T ;

Ostrowski, R ;

Dott, W .

CHEMOSPHERE, 1999, 38 (08) :1745-1755

← 1 2 3 4 →