Synthesis and application of molecularly imprinted polymers for trypsin piezoelectric sensors

被引：48

作者：

Karaseva, Nadezhda A. ^{[1
,2
]}

Pluhar, Bettina ^{[1
]}

Beliaeva, Ekaterina A. ^{[2
]}

Ermolaeva, Tatyana N. ^{[2
]}

Mizaikoff, Boris ^{[1
]}

机构：

[1] Ulm Univ, Inst Analyt & Bioanalyt Chem, D-89081 Ulm, Germany

[2] Lipetsk State Tech Univ, Dept Chem, Lipetsk 398600, Russia

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2019年 / 280卷

关键词：

Molecularly imprinted polymer; MIP; Trypsin; Miniemulsion polymerization; Piezoelectric sensor; MINIEMULSION POLYMERIZATION; NANOPARTICLES; ANTIBIOTICS;

D O I：

10.1016/j.snb.2018.10.022

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Polymer particles imprinted for the protein trypsin (MIPs) were synthesized via miniemulsion polymerization. The influence of the nature of the cross-linker and the incubation time on the characteristics of the polymer particles was investigated. Optimized results concerning binding capacity and selectivity were obtained for MIPs utilizing methacrylic acid and ethylene glycol dimethacrylate for generating MIP particles with a diameter of 200 nm. It was found that thus obtained materials follow pseudo-second order sorption kinetics when rebinding the template. These MIP particles were then used as molecular recognition element for biomimetic piezoelectric sensors directly assaying trypsin. The obtained calibration functions corroborated a linear response in a concentration range of 0.125-2 mu g mL(-1) with a limit of detection at 0.07 mu g mL(-1). Finally, the developed sensor was tested for the detection of trypsin in pharmaceutical formulations.

引用

页码：272 / 279

页数：8

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