Diagnostic and prognostic value of the cancer-testis antigen lactate dehydrogenase C4 in breast cancer

被引：41
作者：
Cui, Zhaolei [1 ]
Chen, Yansong [1 ]
Hu, Minhua [1 ]
Lin, Yingfeng [1 ]
Zhang, Shuyu [1 ]
Kong, Lingying [2 ]
Chen, Yan [1 ]
机构：
[1] Fujian Med Univ, Dept Clin Lab, Lab Biochem & Mol Biol Res, Fujian Prov Key Lab Tumor Biotherapy,Canc Hosp, 420 Fuma Rd, Fuzhou 350014, Fujian, Peoples R China
[2] Fujian Univ Tradit Chinese Med, Dept Pathol, Affiliated Peoples Hosp, 602 Middle Rd, Fuzhou 350004, Fujian, Peoples R China
来源：
CLINICA CHIMICA ACTA | 2020年 / 503卷
基金：
中国国家自然科学基金;
关键词：
Lactate dehydrogenase C4; Breast cancer; Serum; Exosome; Diagnosis; Prognosis; TUMOR INVASION; EXPRESSION; STATISTICS; GENE;
D O I：
10.1016/j.cca.2019.11.032
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background: Lactate dehydrogenase C4 (LDH-C4) as a cancer/testis antigen (CTA) is abnormally expressed in some malignant tumors. However, the expression and clinical significance of LDH-C4 in breast cancer (BC) has not been characterized. Methods: We determined LDHC mRNA expression in serum and serum-derived exosomes of BC patients by quantitative RT-PCR. We also evaluated the protein expression of LDH-C4 in BC tissues using high-throughput tissue microarray analysis and immunohistochemistry. Results: Our results showed high mRNA expression level of LDHC in serum and serum-derived exosomes of BC patients. The LDHC level in serum and exosomes could distinguish BC cases from healthy individuals based on their AUCs of 0.9587 and 0.9464, respectively. Besides, the LDHC level in exosomes of BC patients associated with tumor size, and positively correlated with HER2 and Ki-67 expressions (all with P < 0.05). Serum and exosomal level of LDHC negatively correlated with medical treatment and positively with the recurrence of BC. Survival analysis showed that LDH-C4 expression negatively correlated with BC prognosis. Conclusion: Serum and exosomal LDHC may be an effective indicator for the diagnosis, efficacy evaluation, and monitoring the recurrence of BC. LDH-C4 may act as a biomarker that predicts BC prognosis.
引用
收藏
页码：203 / 209
页数：7
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