Use of Adeno-associated viral vectors to improve delivery of a DNA vaccine against dengue virus

被引：6

作者：

Slon-Campos, Jose Luis ^{[1
,2
]}

Poggianella, Monica ^{[1
]}

Zentilin, Lorena ^{[1
]}

Burrone, Oscar R. ^{[1
]}

机构：

[1] ICGEB, Padriciano 99, I-34149 Trieste, Italy

[2] Univ Oxford, Wellcome Ctr Human Genet, Nuffield Dept Med, Roosevelt Dr, Oxford OX3 7BN, England

来源：

JOURNAL OF GENERAL VIROLOGY | 2020年 / 101卷 / 01期

关键词：

Dengue; flavivirus; DNA vaccines; viral-vectored vaccines; adeno-associated virus; dengue virus; IMMUNE-RESPONSE; ENVELOPE GLYCOPROTEIN; DENDRITIC CELLS; GENE-THERAPY; TRANSDUCTION; INFECTION; SEROTYPE; ANTIBODIES; SAFETY; AAV;

D O I：

10.1099/jgv.0.001351

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Dengue virus (DENV) remains a significant healthcare and socioeconomic burden for endemic countries. Attempts to produce a safe and effective vaccine have been unsuccessful so far, making this task one of the top priorities in the field. We have previously shown that an EOM-based DNA vaccine is able to induce neutralizing, long-lasting and highly specific antibody responses for all four DENV serotypes in mice using gene-gun delivery technology. Here, we describe the use of recombinant Adeno-associated viral vectors as an alternative DNA delivery platform, in combination with different immunization schedules, to simplify the vaccination protocol without compromising the induction of neutralizing antibody responses. Our results demonstrate that using viral vectored-platforms to deliver genetic vaccines could potentially reduce the number of doses required to induce a sustained DENV-neutralizing response, thus facilitating the implementation and deployment of the vaccine in developing countries.

引用

收藏

页码：73 / 78

页数：6

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