Pyrene-perylene as a FRET pair coupled to the N2′-functionality of 2′-amino-LNA

被引：45
|
作者：
Lindegaard, Dorthe [1 ]
Madsen, Andreas S. [1 ]
Astakhova, Irina V. [2 ]
Malakhov, Andrei D. [2 ]
Babu, B. Ravindra [1 ]
Korshun, Vladimir A. [2 ]
Wengel, Jesper [1 ]
机构：
[1] Univ So Denmark, Nucl Acid Ctr, DK-5230 Odense, Denmark
[2] Russian Acad Sci, Shemyakin Ouchinnilov Inst Bioorgan Chem, Moscow 117997, Russia
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 01期
基金：
俄罗斯基础研究基金会;
关键词：
D O I：
10.1016/j.bmc.2007.04.056
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl NT-functionalized 2'-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40 A apart to approximately 90% when the fluorophores were in close proximity. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：94 / 99
页数：6
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