Ultrahigh-contrast imaging by temporally modulated stimulated emission depletion

被引：14

作者：

Doronina-Amitonova, L. V. ^{[1
,2
]}

Fedotov, I. V. ^{[1
,2
,3
]}

Zheltikov, A. M. ^{[1
,2
,3
,4
]}

机构：

[1] Moscow MV Lomonosov State Univ, Ctr Int Laser, Dept Phys, Moscow 119992, Russia

[2] Russian Quantum Ctr, Skolkovo 143025, Moscow Region, Russia

[3] Texas A&M Univ, Dept Phys & Astron, College Stn, TX 77843 USA

[4] Kurchatov Inst, Natl Res Ctr, Moscow 123182, Russia

来源：

OPTICS LETTERS | 2015年 / 40卷 / 05期

基金：

俄罗斯科学基金会; 俄罗斯基础研究基金会;

关键词：

NITROGEN-VACANCY CENTERS; MAGNETIC-FIELD; SPIN; MICROSCOPY;

D O I：

10.1364/OL.40.000725

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Stimulated emission depletion (STED) is the key optical technology enabling super-resolution microscopy below the diffraction limit. Here, we demonstrate that modulation of STED in the time domain, combined with properly designed lock-in detection, can radically enhance the contrast of fluorescent images of strongly autofluorescent biotissues. In our experiments, the temporally modulated STED technique, implemented with low-intensity continuous- wave laser sources, is shown to provide an efficient all-optical suppression of a broadband fluorescent background, allowing the contrast of fluorescent images of mammal brain tissues tagged with nitrogen-vacancy diamond to be increased by five orders of magnitude. (C) 2015 Optical Society of America

引用

页码：725 / 728

页数：4

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