IGF-I promotes Schwann cell motility and survival via activation of Akt

被引：54
作者：
Cheng, HL
Steinway, M
Delaney, CL
Franke, TF
Feldman, EL
机构：
[1] Univ Michigan, Dept Neurol, Ann Arbor, MI 48109 USA
[2] Columbia Univ, Dept Pharmacol, New York, NY 10032 USA
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2000年 / 170卷 / 1-2期
关键词：
IGF-I; Schwann cell; Akt; motility; apoptosis;
D O I：
10.1016/S0303-7207(00)00324-5
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We previously reported insulin-like growth factor-I (IGF I) promotes Schwann cell (SC) motility and rescues SC from apoptosis induced by serum deprivation. This effect is mediated by phosphatidylinositol-3 (PI-3) kinase. In the current study, we examined the role of Akt, a downstream kinase of PI-3K, in SC motility and IGF I mediated protection from apoptosis. IGF-I induces Akt phosphorylation at Ser473, an event which may be blocked by pretreatment with a PI-3K inhibitor, LY294002. In dominant negative K179M Akt (K179M) transfected SC, however, Akt is not activated in response to IGF-I. In addition, ICF-I is unable to promote SC motility and survival in K179M SC. These results suggest a critical role for Akt in IGF-I mediated motility and survival in SC. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：211 / 215
页数：5
相关论文
共 24 条
  • [1] Mechanism of activation of protein kinase B by insulin and IGF-1
    Alessi, DR
    Andjelkovic, M
    Caudwell, B
    Cron, P
    Morrice, N
    Cohen, P
    Hemmings, BA
    [J]. EMBO JOURNAL, 1996, 15 (23) : 6541 - 6551
  • [2] Blair LAC, 1999, J NEUROSCI, V19, P1940
  • [3] STUDIES ON CULTURED RAT SCHWANN-CELLS .1. ESTABLISHMENT OF PURIFIED POPULATIONS FROM CULTURES OF PERIPHERAL-NERVE
    BROCKES, JP
    FIELDS, KL
    RAFF, MC
    [J]. BRAIN RESEARCH, 1979, 165 (01) : 105 - 118
  • [4] Campana WM, 1999, J NEUROSCI RES, V57, P332
  • [5] IGF-I promotes peripheral nervous system myelination
    Cheng, HL
    Russell, JW
    Feldman, EL
    [J]. CHARCOT-MARIE-TOOTH DISORDERS, 1999, 883 : 124 - 130
  • [6] Cheng HL, 1996, J NEUROCHEM, V66, P525
  • [7] Regulation of insulin-like growth factor-binding protein-5 expression during Schwann cell differentiation
    Cheng, HL
    Shy, M
    Feldman, EL
    [J]. ENDOCRINOLOGY, 1999, 140 (10) : 4478 - 4485
  • [8] Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery
    Datta, SR
    Dudek, H
    Tao, X
    Masters, S
    Fu, HA
    Gotoh, Y
    Greenberg, ME
    [J]. CELL, 1997, 91 (02) : 231 - 241
  • [9] Delaney CL, 1999, J NEUROBIOL, V41, P540, DOI 10.1002/(SICI)1097-4695(199912)41:4<540::AID-NEU9>3.0.CO
  • [10] 2-P
123