Differential Expression Pattern of Exosome Long Non-Coding RNAs (lncRNAs) and MicroRNAs (miRNAs) in Vascular Endothelial Cells Under Heat Stroke

被引:31
作者
Chen, Huai-Sheng [1 ,2 ]
Tong, Hua-Sheng [1 ]
Zhao, Ying [2 ]
Hong, Cheng-Ying [2 ]
Bin, Jian-Ping [3 ]
Su, Lei [1 ]
机构
[1] Southern Med Univ, Guangzhou Sch Clin Med, Dept Crit Care Med, Guangzhou Gen Hosp Guangzhou Mil Reg, Guangzhou, Guangdong, Peoples R China
[2] Jinan Univ, Dept Crit Care Med, Shenzhen Peoples Hosp, Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China
[3] Southern Med Univ, Southern Affiliated Hosp, Dept Cardiovasc Dis, Guangzhou, Guangdong, Peoples R China
来源
MEDICAL SCIENCE MONITOR | 2018年 / 24卷
关键词
Endothelial Cells; Exosomes; Heat Stroke; MicroRNAs; RNA; Long Noncoding; DISSEMINATED INTRAVASCULAR COAGULATION; ORGANOTROPIC METASTASIS; P53; ACTIVATION; APOPTOSIS; PATHWAYS; STRESS; INJURY; MODEL; MICE; RATS;
D O I
10.12659/MSM.909983
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background: Heat stroke is a life-threatening disease which is characterized by a high body temperature and multiple organ dysfunction syndrome. Vascular endothelial cell injury is a main feature of heat stroke. Little is known about the long noncoding RNA (lncRNA) and microRNA (miRNA) expression alternation in endothelial cell exosomes related to heat stroke. The aim of this study was to explore the changes of lncRNAs and miRNAs expression pattern in exosomes derived from vascular endothelial cells under heat stroke temperature conditions. Material/Methods: Cultured medium exosomes from HUVECs (human vascular endothelial cells) either under normal temperature or heat stroke temperature conditions were harvested; then RNA was extracted and the lncRNAs and miRNAs were analyzed by high throughput sequencing. Results: Ten significantly upregulated and 10 downregulated lncRNAs were identified in exosomes derived from heat stroke temperature treated cells. Furthermore, GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses were used to evaluate the signaling pathway of differential expressions in lncRNAs. Finally, the interaction network of lncRNAs-miRNAs-mRNA was uncovered using ceRNA (competing endogenous RNA) principle via prediction software. Conclusions: These results indicate that the identified lncRNAs and miRNAs in endothelial cell exosomes might serve as non-invasive biomarkers for heat stroke.
引用
收藏
页码:7965 / 7974
页数:10
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