Intracellular interference of infectious bursal disease virus

被引：4

作者：

González, D ^{[1
]}

Rodríguez, JF ^{[1
]}

Abaitua, F ^{[1
]}

机构：

[1] CSIC, Dept Biol Mol & Celular, Ctr Nacl Biotecnol, E-28049 Madrid, Spain

来源：

JOURNAL OF VIROLOGY | 2005年 / 79卷 / 22期

关键词：

D O I：

10.1128/JVI.79.22.14437-14441.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A search for dominant-negative mutant polypeptides hampering infectious bursal disease virus (IBDV) replication has been undertaken. We have found that expression of a mutant version of the VP3 structural polypeptide known as VP3/M3, partially lacking the domain responsible for the interaction with the virus-encoded RNA polymerase, efficiently interferes with the IBDV replication cycle. Transformed cells stably expressing VP3/M3 show a significant reduction (up to 96%) in their ability to support IBDV growth. Our findings provide a new tool for the characterization of the IBDV replication cycle and might facilitate the generation of genetically modified chicken lines with a reduced susceptibility to IBDV infection.

引用

页码：14437 / 14441

页数：5

共 16 条

[1] Baculovirus-mediated gene transfer into mammalian cells [J].

Boyce, FM ;

Bucher, NLR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (06) :2348-2352

[2] GENETIC-DIFFERENCES IN SUSCEPTIBILITY OF CHICKEN LINES TO INFECTION WITH INFECTIOUS BURSAL DISEASE VIRUS [J].

BUMSTEAD, N ;

REECE, RL ;

COOK, JKA .

POULTRY SCIENCE, 1993, 72 (03) :403-410

[3]

FernandezArias A, 1997, J VIROL, V71, P8014

[4] Characterization of the RNA-binding activity of VP3, a major structural protein of Infectious bursal disease virus [J].

Kochan, G ;

Gonzalez, D ;

Rodriguez, JF .

ARCHIVES OF VIROLOGY, 2003, 148 (04) :723-744

[5] VP1, the putative RNA-dependent RNA polymerase of infectious bursal disease virus, forms complexes with the capsid protein VP3, leading to efficient encapsidation into virus-like particles [J].

Lombardo, E ;

Maraver, A ;

Castón, JR ;

Rivera, J ;

Fernández-Arias, A ;

Serrano, A ;

Carrascosa, JL ;

Rodriguez, JF .

JOURNAL OF VIROLOGY, 1999, 73 (08) :6973-6983

[6] The oligomerization domain of VP3, the scaffolding protein of infectious bursal disease virus, plays a critical role in capsid assembly [J].

Maraver, A ;

Oña, A ;

Abaitua, F ;

González, D ;

Clemente, R ;

Ruiz-Díaz, JA ;

Castón, JR ;

Pazos, F ;

Rodriguez, JF .

JOURNAL OF VIROLOGY, 2003, 77 (11) :6438-6449

[7] Identification and molecular characterization of the RNA polymerase-binding motif of infectious bursal disease virus inner capsid protein VP3 [J].

Maraver, A ;

Clemente, R ;

Rodríguez, JF ;

Lombardo, E .

JOURNAL OF VIROLOGY, 2003, 77 (04) :2459-2468

[8] Different architectures in the assembly of infectious bursal disease virus capsid proteins expressed in insect cells [J].

Martinez-Torrecuadrada, JL ;

Castón, JR ;

Castro, M ;

Carrascosa, JL ;

Rodriguez, JF ;

Casal, JI .

VIROLOGY, 2000, 278 (02) :322-331

[9] Efficient production of germline transgenic chickens using lentiviral vectors [J].

McGrew, MJ ;

Sherman, A ;

Ellard, FM ;

Lillico, SG ;

Gilhooley, HJ ;

Kingsman, AJ ;

Mitrophanous, KA ;

Sang, H .

EMBO REPORTS, 2004, 5 (07) :728-733

[10] The C-terminal domain of the pVP2 precursor is essential for the interaction between VP2 and VP3, the capsid polypeptides of infectious bursal disease virus [J].

Oña, A ;

Luque, D ;

Abaitua, F ;

Maraver, A ;

Castón, JR ;

Rodríguez, JF .

VIROLOGY, 2004, 322 (01) :135-142

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