TILLING in extremis

被引：89

作者：

Wang, Trevor L. ^{[1
]}

Uauy, Cristobal ^{[2
]}

Robson, Fran ^{[1
]}

Till, Brad ^{[3
]}

机构：

[1] John Innes Ctr Plant Sci Res, RevGenUK, Norwich NR4 7UH, Norfolk, England

[2] Natl Inst Agr Bot, Cambridge, England

[3] IAEA, Div Nucl Techn Food & Agr, Plant Breeding & Genet Lab,Vienna Int Ctr, Joint FAO IAEA Div Nucl Techn Food & Agr, A-1400 Vienna, Austria

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2012年 / 10卷 / 07期

基金：

英国生物技术与生命科学研究理事会;

关键词：

mutation discovery; next-generation sequencing; plant breeding; polyploids; TILLING; vegetatively propagated crops; CHEMICALLY-INDUCED MUTATIONS; DETECT INDUCED MUTATIONS; INDUCED POINT MUTATIONS; REVERSE GENETICS; LOTUS-JAPONICUS; FUNCTIONAL GENOMICS; NUCLEOTIDE POLYMORPHISMS; MEDICAGO-TRUNCATULA; ALLELIC SERIES; DISCOVERY;

D O I：

10.1111/j.1467-7652.2012.00708.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Targeting induced local lesions in genomes (TILLING), initially a functional genomics tool in model plants, has been extended to many plant species and become of paramount importance to reverse genetics in crops species. Because it is readily applicable to most plants, it remains a dominant non-transgenic method for obtaining mutations in known genes. The process has seen many technological changes over the last 10 years; a major recent change has been the application of next-generation sequencing (NGS) to the process, which permits multiplexing of gene targets and genomes. NGS will ultimately lead to TILLING becoming an in silico procedure. We review here the history and technology in brief, but focus more importantly on recent developments in polyploids, vegetatively propagated crops and the future of TILLING for plant breeding.

引用

页码：761 / 772

页数：12

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