SEDS proteins are a widespread family of bacterial cell wall polymerases

被引:348
作者
Meeske, Alexander J. [1 ]
Riley, Eammon P. [1 ]
Robins, William P. [1 ]
Uehara, Tsuyoshi [1 ]
Mekalanos, John J. [1 ]
Kahne, Daniel [2 ,3 ]
Walker, Suzanne [1 ,2 ,3 ]
Kruse, Andrew C. [2 ]
Bernhardt, Thomas G. [1 ]
Rudner, David Z. [1 ]
机构
[1] Harvard Med Sch, Dept Microbiol & Immunobiol, 77 Ave Louis Pasteur, Boston, MA 02115 USA
[2] Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, 250 Longwood Ave, Boston, MA 02115 USA
[3] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
关键词
PENICILLIN-BINDING PROTEINS; BETA-LACTAM RESISTANCE; BACILLUS-SUBTILIS; PEPTIDOGLYCAN SYNTHESIS; DIVISION; GENE; BIOSYNTHESIS; PRECURSORS; DYNAMICS; FLIPPASE;
D O I
10.1038/nature19331
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Elongation of rod-shaped bacteria is mediated by a dynamic peptidoglycan-synthetizing machinery called the Rod complex. Here we report that, in Bacillus subtilis, this complex is functional in the absence of all known peptidoglycan polymerases. Cells lacking these enzymes survive by inducing an envelope stress response that increases the expression of RodA, a widely conserved core component of the Rod complex. RodA is a member of the SEDS (shape, elongation, division and sporulation) family of proteins, which have essential but ill-defined roles in cell wall biogenesis during growth, division and sporulation. Our genetic and biochemical analyses indicate that SEDS proteins constitute a family of peptidoglycan polymerases. Thus, B. subtilis and probably most bacteria use two distinct classes of polymerase to synthesize their exoskeleton. Our findings indicate that SEDS family proteins are core cell wall synthases of the cell elongation and division machinery, and represent attractive targets for antibiotic development.
引用
收藏
页码:634 / +
页数:15
相关论文
共 47 条
[1]   Degradation and reconstruction of moenomycin A and derivatives: Dissecting the function of the isoprenoid chain [J].
Adachi, Masaatsu ;
Zhang, Yi ;
Leimkuhler, Catherine ;
Sun, Binyuan ;
LaTour, John V. ;
Kahne, Daniel E. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (43) :14012-14013
[2]   Role of class A penicillin-binding proteins in PBP5-mediated β-lactam resistance in Enterococcus faecalis [J].
Arbeloa, A ;
Segal, H ;
Hugonnet, JE ;
Josseaume, N ;
Dubost, L ;
Brouard, JP ;
Gutmann, L ;
Mengin-Lecreulx, D ;
Arthur, M .
JOURNAL OF BACTERIOLOGY, 2004, 186 (05) :1221-1228
[3]   Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants:: the Keio collection [J].
Baba, Tomoya ;
Ara, Takeshi ;
Hasegawa, Miki ;
Takai, Yuki ;
Okumura, Yoshiko ;
Baba, Miki ;
Datsenko, Kirill A. ;
Tomita, Masaru ;
Wanner, Barry L. ;
Mori, Hirotada .
MOLECULAR SYSTEMS BIOLOGY, 2006, 2 (1) :2006.0008
[4]   A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis [J].
Daniel, RA ;
Williams, AM ;
Errington, J .
JOURNAL OF BACTERIOLOGY, 1996, 178 (08) :2343-2350
[5]   Processive Movement of MreB-Associated Cell Wall Biosynthetic Complexes in Bacteria [J].
Dominguez-Escobar, Julia ;
Chastanet, Arnaud ;
Crevenna, Alvaro H. ;
Fromion, Vincent ;
Wedlich-Soeldner, Roland ;
Carballido-Lopez, Rut .
SCIENCE, 2011, 333 (6039) :225-228
[6]   The Bacillus subtilis σM regulon and its contribution to cell envelope stress responses [J].
Eiamphungporn, Warawan ;
Helmann, John D. .
MOLECULAR MICROBIOLOGY, 2008, 67 (04) :830-848
[7]   Interactions Between Late-Acting Proteins Required for Peptidoglycan Synthesis during Sporulation [J].
Fay, Allison ;
Meyer, Pablo ;
Dworkin, Jonathan .
JOURNAL OF MOLECULAR BIOLOGY, 2010, 399 (04) :547-561
[8]   The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex in Escherichia coli [J].
Fraipont, Claudine ;
Alexeeva, Svetlana ;
Wolf, Benoit ;
van der Ploeg, Rene ;
Schloesser, Marie ;
den Blaauwen, Tanneke ;
Nguyen-Disteche, Martine .
MICROBIOLOGY-SGM, 2011, 157 :251-259
[9]   Temporal and selective association of multiple sigma factors with RNA polymerase during sporulation in Bacillus subtilis [J].
Fujita, M .
GENES TO CELLS, 2000, 5 (02) :79-88
[10]   Two-Step Assembly Dynamics of the Bacillus subtilis Divisome [J].
Gamba, Pamela ;
Veening, Jan-Willem ;
Saunders, Nigel J. ;
Hamoen, Leendert W. ;
Daniel, Richard A. .
JOURNAL OF BACTERIOLOGY, 2009, 191 (13) :4186-4194