Glycosidases - a great synthetic tool

被引：89

作者：

Scigelova, M ^{[1
]}

Singh, S ^{[1
]}

Crout, DHG ^{[1
]}

机构：

[1] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 1999年 / 6卷 / 05期

关键词：

glycosidases; screening; enzymatic glycosylation; oligosaccharide synthesis;

D O I：

10.1016/S1381-1177(99)00012-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycosidases were used to prepare oligosaccharide structures of physiological and medicinal relevance. The study included an extensive screening of crude enzymatic preparations for alpha- and beta-galactosidase, alpha- and beta-mannosidase, beta-N-acetylglucosaminidase, beta-N-acetylgalactosaminidase and alpha-L-fucosidase activities. The enzymes were assessed with respect to regioselectivity of glycosyl transfer on to carbohydrate accepters. The purification procedures for individual biocatalysts are described in detail. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：483 / 494

页数：12

共 21 条

[1] An α-L-fucosidase from Penicillium multicolor as a candidate enzyme for the synthesis of a (1→3)-linked fucosyl oligosaccharides by transglycosylation [J].

Ajisaka, K ;

Fujimoto, H ;

Miyasato, M .

CARBOHYDRATE RESEARCH, 1998, 309 (01) :125-129

[2] ENZYMATIC-SYNTHESIS OF MANNOBIOSES AND MANNOTRIOSES BY REVERSE HYDROLYSIS USING ALPHA-MANNOSIDASE FROM ASPERGILLUS-NIGER [J].

AJISAKA, K ;

MATSUO, I ;

ISOMURA, M ;

FUJIMOTO, H ;

SHIRAKABE, M ;

OKAWA, M .

CARBOHYDRATE RESEARCH, 1995, 270 (02) :123-130

[3]

[Anonymous], UNPUB

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] A microorganism decomposing group-specific a substances [J].

Chase, MW .

JOURNAL OF BACTERIOLOGY, 1938, 36 (04) :383-390

[6] A CONVERGENT AND STEREOCONTROLLED SYNTHETIC ROUTE TO THE CORE PENTASACCHARIDE STRUCTURE OF ASPARAGINE-LINKED GLYCOPROTEINS [J].

DAN, A ;

ITO, Y ;

OGAWA, T .

JOURNAL OF ORGANIC CHEMISTRY, 1995, 60 (15) :4680-4681

[7] Purification and properties of recombinant β-galactosidase from Bacillus circulans [J].

Fujimoto, H ;

Miyasato, M ;

Ito, Y ;

Sasaki, T ;

Ajisaka, K .

GLYCOCONJUGATE JOURNAL, 1998, 15 (02) :155-160

[8] Microbial alpha-mannosidases [J].

Keskar, SS ;

Gaikwad, SM ;

Khan, MI .

ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (08) :602-604

[9] ENZYMATIC SYNTHESES OF N-ACETYLLACTOSAMINE AND N-ACETYLALLOLACTOSAMINE BY THE USE OF BETA-D-GALACTOSIDASES [J].

SAKAI, K ;

KATSUMI, R ;

OHI, H ;

USUI, T ;

ISHIDO, Y .

JOURNAL OF CARBOHYDRATE CHEMISTRY, 1992, 11 (05) :553-565

[10]

SCIGELOVA M, 1997, THESIS CHARLES U PRA

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