Expression of atrC -: encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans -: is sensitive to cycloheximide

被引：23
作者：
Angermayr, K
Parson, W
Stöffler, G
Haas, H
机构：
[1] Univ Innsbruck, Sch Med, Dept Microbiol, A-6020 Innsbruck, Austria
[2] Univ Innsbruck, Sch Med, Inst Forens Med, A-6020 Innsbruck, Austria
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 1999年 / 1453卷 / 02期
关键词：
ABC transporter; cycloheximide; (Aspergillus nidulans);
D O I：
10.1016/S0925-4439(98)00107-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided. (C) 1999 Elsevier Science B.V. All rights reserved.
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收藏
页码：304 / 310
页数：7
相关论文
共 35 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]  
[Anonymous], INTELLIG SYST MOL BI
[3]   CHROMOSOME-SPECIFIC RECOMBINANT-DNA LIBRARIES FROM THE FUNGUS ASPERGILLUS-NIDULANS [J].
BRODY, H ;
GRIFFITH, J ;
CUTICCHIA, AJ ;
ARNOLD, J ;
TIMBERLAKE, WE .
NUCLEIC ACIDS RESEARCH, 1991, 19 (11) :3105-3109
[4]   THE ASPERGILLUS-NIDULANS MITOCHONDRIAL GENOME [J].
BROWN, TA ;
WARING, RB ;
SCAZZOCCHIO, C ;
DAVIES, RW .
CURRENT GENETICS, 1985, 9 (02) :113-117
[5]   THE MITOCHONDRIAL URF1 GENE IN NEUROSPORA-CRASSA HAS AN INTRON THAT CONTAINS A NOVEL TYPE OF URF [J].
BURGER, G ;
WERNER, S .
JOURNAL OF MOLECULAR BIOLOGY, 1985, 186 (02) :231-242
[6]   INTERNAL DUPLICATION AND HOMOLOGY WITH BACTERIAL TRANSPORT PROTEINS IN THE MDR1 (P-GLYCOPROTEIN) GENE FROM MULTIDRUG-RESISTANT HUMAN-CELLS [J].
CHEN, CJ ;
CHIN, JE ;
UEDA, K ;
CLARK, DP ;
PASTAN, I ;
GOTTESMAN, MM ;
RONINSON, IB .
CELL, 1986, 47 (03) :381-389
[7]   Isolation and characterisation of cycloheximide sensitive mutants of Aspergillus nidulans [J].
de Souza, CC ;
Pellizzon, CH ;
Hiraishi, M ;
Goldman, MHS ;
Goldman, GH .
CURRENT GENETICS, 1998, 33 (01) :60-69
[8]   Complete inventory of the yeast ABC proteins [J].
Decottignies, A ;
Goffeau, A .
NATURE GENETICS, 1997, 15 (02) :137-145
[9]   IDENTIFICATION AND CHARACTERIZATION OF SNQ2, A NEW MULTIDRUG ATP BINDING CASSETTE TRANSPORTER OF THE YEAST PLASMA-MEMBRANE [J].
DECOTTIGNIES, A ;
LAMBERT, L ;
CATTY, P ;
DEGAND, H ;
EPPING, EA ;
MOYEROWLEY, WS ;
BALZI, E ;
GOFFEAU, A .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (30) :18150-18157
[10]   Multidrug resistance in Aspergillus nidulans involves novel ATP-binding cassette transporters [J].
Del Sorbo G. ;
Andrade A.C. ;
Van Nistelrooy J.G.M. ;
Van Kan J.A.L. ;
Balzi E. ;
De Waard M.A. .
Molecular and General Genetics MGG, 1997, 254 (4) :417-426
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