Use of quantitative PCR to measure density of Borrelia burgdorferi in the midgut and salivary glands of feeding tick vectors

被引：119

作者：

Piesman, J ^{[1
]}

Schneider, BS ^{[1
]}

Zeidner, NS ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div Vector Borne Infect Dis, Natl Ctr Infect Dis, US PHS, Ft Collins, CO 80522 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 11期

关键词：

D O I：

10.1128/JCM.39.11.4145-4148.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Quantitative real-time PCR was used to assay spirochetes in feeding ticks. Spirochetes in tick midguts increased sixfold, from 998 per tick before attachment to 5,884 at 48 h of attachment. Spirochetes in tick salivary glands increased > 17-fold, from 1.2 per salivary gland pair before feeding to 20.8 at 72 h postattachment. The period of the most rapid increase in the number of spirochetes in the salivary glands occurred from 48 to 60 h postattachment; this time period coincides with the maximal increase in transmission risk during nymphal tick feeding.

引用

页码：4145 / 4148

页数：4

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