Baicalin alleviates oxidative stress damage in trabecular meshwork cells in vitro

被引:22
作者
Gong, Lei [1 ]
Zhu, Jianfeng [2 ]
机构
[1] Jinan Eighth Peoples Hosp, Dept Ophthalmol, 73 Wenhua Dong Rd, Jinan 250000, Shandong, Peoples R China
[2] Linyi Peoples Hosp, Dept Ophthalmol, 48 Jiefang Rd, Linyi 276000, Shandong, Peoples R China
关键词
Baicalin; Glaucoma; Trabecular meshwork cells; Oxidative stress; Inflammation; HYDROGEN-PEROXIDE; INFLAMMATORY CYTOKINES; CANCER-CELLS; APOPTOSIS; GLAUCOMA; ANTIOXIDANTS; INHIBITION; INJURY; VIRUS; DEATH;
D O I
10.1007/s00210-017-1433-9
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Oxidative stress and inflammation play a key role in pathophysiology of glaucoma. Baicalin is known as an anti-oxidative and anti-inflammatory substance, possessing the potential to treat glaucoma. This study was designed to assess the anti-oxidative and anti-inflammatory potentials in culture human trabecular meshwork (hTM) cells. Using hTM cells as the in vitro model, we investigated the effects of baicalin on oxidative stress-induced markers for hTM impairments. We pre-incubated hTM cells with baicalin before hydrogen peroxide stressing or pre- plus co-incubated with baicalin before and during stressing and monitored the cell death, production of intracellular reactive oxygen species (iROS) and inflammatory cytokines, accumulation of carbonylated proteins, and activity of senescence marker. Samples that received pre- plus co-treatment with 10 or 15 mu M baicalin showed significantly increased cell survival and decreased iROS production. Further studies demonstrated that pre- plus co-treatment with 15 mu M baicalin significantly inhibited proinflammatory factor IL-1 alpha and ELAM-1 production, decreased activities of senescence marker SA-beta-gal, and lowered carbonylated protein levels. In contrast, samples that received only pre-treatment did not show any of these protective effects. Baicalin can protect hTM cells against oxidative stress, shedding light on potential treatment for glaucoma.
引用
收藏
页码:51 / 58
页数:8
相关论文
共 31 条
  • [1] Antioxidants Protect Trabecular Meshwork Cells From Hydrogen Peroxide-Induced Cell Death
    Ammar, David A.
    Hamweyah, Karam M.
    Kahook, Malik Y.
    [J]. TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, 2012, 1 (01):
  • [2] INHIBITION OF HUMAN T-CELL LEUKEMIA-VIRUS BY THE PLANT FLAVONOID BAICALIN (7-GLUCURONIC ACID, 5,6-DIHYDROXYFLAVONE)
    BAYLOR, NW
    FU, T
    YAN, YD
    RUSCETTI, FW
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1992, 165 (03) : 433 - 437
  • [3] Inhibitory Effect of Baicalin and Baicalein on Ovarian Cancer Cells
    Chen, Jianchu
    Li, Zhaoliang
    Chen, Allen Y.
    Ye, Xingqian
    Luo, Haitao
    Rankin, Gary O.
    Chen, Yi Charlie
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (03) : 6012 - 6025
  • [4] Chua J, 2012, MOL VIS, V18, P431
  • [5] Erb Carl, 2011, Front Biosci (Elite Ed), V3, P1524
  • [6] Famili A, 2013, MOL VIS, V19, P1304
  • [7] Protective effects of flavonoids in the roots of Scutellaria baicalensis Georgi against hydrogen peroxide-induced oxidative stress in HS-SY5Y cells
    Gao, ZH
    Huang, KX
    Xu, HB
    [J]. PHARMACOLOGICAL RESEARCH, 2001, 43 (02) : 173 - 178
  • [8] Mitochondrial complex I defect induces ROS release and degeneration in trabecular meshwork cells of POAG patients: Protection by antioxidants
    He, Yuan
    Leung, Kar Wah
    Zhang, Yue-Hong
    Duan, Shan
    Zhong, Xiu-Feng
    Jiang, Ru-Zhang
    Peng, Zhan
    Tombran-Tink, Joyce
    Ge, Jian
    [J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2008, 49 (04) : 1447 - 1458
  • [9] Baicalin is a major component of PC-SPES which inhibits the proliferation of human cancer cells via apoptosis and cell cycle arrest
    Ikezoe, T
    Chen, SS
    Heber, D
    Taguchi, H
    Koeffler, HP
    [J]. PROSTATE, 2001, 49 (04) : 285 - 292
  • [10] Methodology for evaluating oxidative DNA damage and metabolic genotypes in human trabecular meshwork
    Izzotti, A
    Cartiglia, C
    De Flora, S
    Saccà, S
    [J]. TOXICOLOGY MECHANISMS AND METHODS, 2003, 13 (03) : 161 - 168