Best practices for cryopreserving, thawing, recovering, and assessing cells

被引：60
作者：
Baust, John M. [1 ,2 ]
Campbell, Lia H. [3 ]
Harbell, John W. [4 ]
机构：
[1] CPSI Biotech, 2 Court St, Owego, NY 13850 USA
[2] SUNY Binghamton, Inst Biomed Technol, Binghamton, NY 13902 USA
[3] Tissue Testing Technol LLC, N Charleston, SC 29406 USA
[4] JHarbell Consulting LLC, 16334 Sunset Valley Dr, Dallas, TX 75248 USA
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 2017年 / 53卷 / 10期
关键词：
Cryopreservation; Freezing; Thawing; Cryoprotectant; Serum; Viability assessment; Apoptosis; Necrosis; Cryopreservation induced delayed onset cell death; Cell function; CD34-POSITIVE STEM-CELLS; LONG-TERM STORAGE; ENHANCED PROTECTION; INDUCED APOPTOSIS; DIVALENT-CATIONS; MAMMALIAN-CELLS; RAT HEPATOCYTES; GENE-EXPRESSION; LIVING CELLS; STABILIZATION;
D O I：
10.1007/s11626-017-0201-y
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Long-term storage of cell stocks insures that cells are available for use whenever needed. Cryopreservation of cells is the method of choice for preservation of important or rare cell stocks. There are several factors to consider when establishing a protocol for freezing, thawing, and recovery of cells after storage. These parameters may include cell concentration, cryoprotectant choice and concentration, and thawing rate among others. Further, the assessment of cell viability and/or function prior to and following cryopreservation is imperative in order to accurately determine downstream utility as well for optimizing the cryopreservation process. This chapter is designed to provide guidance and insight into developing robust and successful protocols for preserving cells that will preserve cell stocks and provide optimal cell yield and viability.
引用
收藏
页码：855 / 871
页数：17
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