Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device

被引：22

作者：

Yamashita, Hitoyoshi ^{[1
,2
]}

Morita, Masamune ^{[2
]}

Sugiura, Haruka ^{[2
]}

Fujiwara, Kei ^{[3
]}

Onoe, Hiroaki ^{[4
]}

Takinoue, Masahiro ^{[2
,5
]}

机构：

[1] Tokyo Inst Technol, Sch Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268502, Japan

[2] Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Computat Intelligence & Syst Sci, Midori Ku, Yokohama, Kanagawa 2268502, Japan

[3] Keio Univ, Dept Biosci & Informat, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan

[4] Keio Univ, Dept Mech Engn, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan

[5] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2015年 / 119卷 / 04期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

Monodisperse microdroplets; Microfluidic device; Centrifuge; Plateau-Rayleigh instability; Biochemical reactions; Cell encapsulation; SINGLE-MOLECULE PCR; HIGH-THROUGHPUT; DROPLETS; MICROREACTORS;

D O I：

10.1016/j.jbiosc.2014.09.018

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report an easy-to-use generation method of biologically compatible monodisperse water-in-oil microdroplets using a glass-capillary-based microfluidic device in a tabletop mini-centrifuge. This device does not require complicated microfabrication; furthermore, only a small sample volume is required in experiments. Therefore, we believe that this method will assist biochemical and cell-biological experiments. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：492 / 495

页数：4

共 6 条

[1] Rapid measurement of fluid viscosity using co-flowing in a co-axial microfluidic device
W. J. Lan
S. W. Li
J. H. Xu
G. S. Luo
Microfluidics and Nanofluidics, 2010, 8 : 687 - 693
[2] Rapid measurement of fluid viscosity using co-flowing in a co-axial microfluidic device
Lan, W. J.
Li, S. W.
Xu, J. H.
Luo, G. S.
MICROFLUIDICS AND NANOFLUIDICS, 2010, 8 (05) : 687 - 693
[3] Multi-chamber microcapsule generation for cell culture using a high- and low-volume centrifuge-based droplet shooting device
Kevin E. Petersen
Abigail E. Asangba
Marina Walther-Antonio
Microfluidics and Nanofluidics, 2023, 27
[4] Multi-chamber microcapsule generation for cell culture using a high- and low-volume centrifuge-based droplet shooting device
Petersen, Kevin E.
Asangba, Abigail E.
Walther-Antonio, Marina
MICROFLUIDICS AND NANOFLUIDICS, 2023, 27 (06)
[5] Generation of disk-like hydrogel beads for cell encapsulation and manipulation using a droplet-based microfluidic device
Kan Liu
Yuliang Deng
Nangang Zhang
Sizhe Li
Huijiang Ding
Feng Guo
Wei Liu
Shishang Guo
Xing-Zhong Zhao
Microfluidics and Nanofluidics, 2012, 13 : 761 - 767
[6] Generation of disk-like hydrogel beads for cell encapsulation and manipulation using a droplet-based microfluidic device
Liu, Kan
Deng, Yuliang
Zhang, Nangang
Li, Sizhe
Ding, Huijiang
Guo, Feng
Liu, Wei
Guo, Shishang
Zhao, Xing-Zhong
MICROFLUIDICS AND NANOFLUIDICS, 2012, 13 (05) : 761 - 767

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