Ag-4-ATP-MWCNT electrode modified with dsDNA as label-free electrochemical sensor for the detection of daunorubicin anticancer drug

被引：27

作者：

Saljooqi, Asma ^{[1
,2
]}

Shamspur, Tayebeh ^{[1
]}

Mostafavi, Ali ^{[1
]}

机构：

[1] Shahid Bahonar Univ Kerman, Dept Chem, POB 76169-133, Kerman, Iran

[2] Shahid Bahonar Univ Kerman, Young Res Soc, POB 76169-133, Kerman, Iran

来源：

BIOELECTROCHEMISTRY | 2017年 / 118卷

关键词：

Ag-4-ATP-MWCNT; dsDNA biosensor; Daunorubicin anticancer drug; Voltammetry; MULTIWALLED CARBON NANOTUBES; DNA-BIOSENSOR; AG NANOPARTICLES; GENE-EXPRESSION; GRAPHENE OXIDE; MICROARRAY; ACID; HYBRIDIZATION; COMPLEXES; BEHAVIOR;

D O I：

10.1016/j.bioelechem.2017.08.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes the synthesis of Ag-4-ATP-MWCNT nanocomposite and its use as a modifier of working electrode. The surface of the electrochemical Ag-4-ATP-MWCNT electrode was modified with a double-stranded DNA (dsDNA) to detect daunorubicin-DNA interactions. Differential pulse voltammetry was applied to develop an electroanalytical procedure for the determination of daunorubicin and evaluate its interaction with dsDNA immobilized on the electrode surface. After the optimization of operational parameters, the linear dependence of the peak current on the daunorubicin concentration was observed in the range of 0.10 x 10(-8) to 1.00 x 10(-5) mol L-1, with the detection and quantification limits of 3.00 x 10(-10) and 1.00 x 10(-9) mol L-1, respectively. The proposed biosensor was successfully applied to validate its capability for the determination of daunorubicin in human serum and urine samples. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：161 / 167

页数：7

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