A library of synthetic transcription activator-like effector-activated promoters for coordinated orthogonal geneexpression in plants

被引：51

作者：

Brueckner, Kathleen ^{[1
]}

Schaefer, Petra ^{[1
]}

Weber, Ernst ^{[2
]}

Gruetzner, Ramona ^{[1
]}

Marillonnet, Sylvestre ^{[1
]}

Tissier, Alain ^{[1
]}

机构：

[1] Leibniz Inst Plant Biochem, Dept Cell & Metab Biol, D-06120 Halle, Saale, Germany

[2] Icon Genet GmbH, D-06120 Halle, Saale, Germany

来源：

PLANT JOURNAL | 2015年 / 82卷 / 04期

关键词：

synthetic promoters; transcription activator-like effectors; orthogonality; metabolic engineering; transient assays; Nicotiana benthamiana; terpenoid; synthetic biology; plants; technical advance; CRISPR-CAS SYSTEMS; III EFFECTORS; NICOTIANA-BENTHAMIANA; BIOSYNTHETIC-PATHWAY; GENE-EXPRESSION; RNA; SEQUENCE; YEAST; ELUCIDATION; INITIATION;

D O I：

10.1111/tpj.12843

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A library of synthetic promoters containing the binding site of a single designer transcription activator-like effector (dTALE) was constructed. The promoters contain a constant sequence, consisting of an 18-base long dTALE-binding site and a TATA box, flanked by degenerate sequences of 49 bases downstream and 19 bases upstream. Forty-three of these promoters were sequenced and tested in transient assays in Nicotiana benthamiana using a GUS reporter gene. The strength of expression of the promoters ranged from around 5% to almost 100% of the viral 35S promoter activity. We then demonstrated the utility of these promoters for metabolic engineering by transiently expressing three genes for the production of a plant diterpenoid in N.benthamiana. The simplicity of the promoter structure shows great promise for the development of genetic circuits, with wide potential applications in plant synthetic biology and metabolic engineering.

引用

页码：707 / 716

页数：10

共 39 条

[1] Promoter engineering: Recent advances in controlling transcription at the most fundamental level [J].

Blazeck, John ;

Alper, Hal S. .

BIOTECHNOLOGY JOURNAL, 2013, 8 (01) :46-+

[2] Rational Diversification of a Promoter Providing Fine-Tuned Expression and Orthogonal Regulation for Synthetic Biology [J].

Blount, Benjamin A. ;

Weenink, Tim ;

Vasylechko, Serge ;

Ellis, Tom .

PLOS ONE, 2012, 7 (03)

[3] Xanthomonas AvrBs3 Family-Type III Effectors: Discovery and Function [J].

Boch, Jens ;

Bonas, Ulla .

ANNUAL REVIEW OF PHYTOPATHOLOGY, VOL 48, 2010, 48 :419-436

[4] Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors [J].

Boch, Jens ;

Scholze, Heidi ;

Schornack, Sebastian ;

Landgraf, Angelika ;

Hahn, Simone ;

Kay, Sabine ;

Lahaye, Thomas ;

Nickstadt, Anja ;

Bonas, Ulla .

SCIENCE, 2009, 326 (5959) :1509-1512

[5]

Brophy JAN, 2014, NAT METHODS, V11, P508, DOI [10.1038/NMETH.2926, 10.1038/nmeth.2926]

[6] High-level diterpene production by transient expression in Nicotiana benthamiana [J].

Brueckner, Kathleen ;

Tissier, Alain .

PLANT METHODS, 2013, 9

[7] From dead leaf, to new life: TAL effectors as tools for synthetic biology [J].

de lange, Orlando ;

Binder, Andreas ;

Lahaye, Thomas .

PLANT JOURNAL, 2014, 78 (05) :753-771

[8] A One Pot, One Step, Precision Cloning Method with High Throughput Capability [J].

Engler, Carola ;

Kandzia, Romy ;

Marillonnet, Sylvestre .

PLOS ONE, 2008, 3 (11)

[9] A Golden Gate Modular Cloning Toolbox for Plants [J].

Engler, Carola ;

Youles, Mark ;

Gruetzner, Ramona ;

Ehnert, Tim-Martin ;

Werner, Stefan ;

Jones, Jonathan D. G. ;

Patron, Nicola J. ;

Marillonnet, Sylvestre .

ACS SYNTHETIC BIOLOGY, 2014, 3 (11) :839-843

[10] Trichome specific expression of the tobacco (Nicotiana sylvestris) cembratrien-ol synthase genes is controlled by both activating and repressing cis-regions [J].

Ennajdaoui, Hanane ;

Vachon, Gilles ;

Giacalone, Cecile ;

Besse, Isabelle ;

Sallaud, Christophe ;

Herzog, Michel ;

Tissier, Alain .

PLANT MOLECULAR BIOLOGY, 2010, 73 (06) :673-685

← 1 2 3 4 →