Avidin-Biotin Cross-Linked Microgel Multilayers as Carriers for Antimicrobial Peptides

被引：18

作者：

Nystrom, Lina ^{[1
]}

Al-Rammahi, Noor ^{[1
]}

Haffner, Sara Malekkhaiat ^{[3
]}

Stromstedt, Adam A. ^{[2
]}

Browning, Kathryn L. ^{[3
]}

Malmsten, Martin ^{[1
,3
]}

机构：

[1] Uppsala Univ, Dept Pharm, SE-75123 Uppsala, Sweden

[2] Uppsala Univ, Dept Med Chem, Pharmacognosy, SE-75123 Uppsala, Sweden

[3] Univ Copenhagen, Dept Pharm, DK-2100 Copenhagen, Denmark

来源：

BIOMACROMOLECULES | 2018年 / 19卷 / 12期

基金：

瑞典研究理事会;

关键词：

BY-LAYER DEPOSITION; INFLAMMATORY RESPONSES; BACTERIAL-COLONIZATION; RELEASE; FILMS; ADSORPTION; RESISTANCE; MEMBRANE; BEHAVIOR;

D O I：

10.1021/acs.biomac.8b01484

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Herein, we report on the formation of crosslinked antimicrobial peptide-loaded microgel multilayers. Poly(ethyl acrylate-co-methacrylic acid) microgels were synthesized and functionalized with biotin to enable the formation of microgel multilayers cross-linked with avidin. Microgel functionalization and avidin cross-linking were verified with infrared spectroscopy, dynamic light scattering, and z-potential measurements, while multilayer formation (up to four layers) was studied with null ellipsometry and quartz crystal microbalance with dissipation (QCM-D). Incorpo-ration of the antimicrobial peptide KYE28 (KYEITTIHNLFRKLTHRLFRRNFGYTLR) into the microgel multilayers was achieved either in one shot after multilayer formation or through addition after each microgel layer deposition. The latter was found to strongly promote peptide incorporation. Further, antimicrobial properties of the peptide-loaded microgel multilayers against Escherichia coli were investigated and compared to those of a peptide-loaded microgel monolayer. Results showed a more pronounced suppression in bacterial viability in suspension for the microgel multilayers. Correspondingly, LIVE/DEAD staining showed promoted disruption of adhered bacteria for the KYE28-loaded multilayers. Taken together, cross-linked microgel multilayers thus show promise as high load surface coatings for antimicrobial peptides.

引用

页码：4691 / 4702

页数：12

共 66 条

[1] Charge based intra-cartilage delivery of single dose dexamethasone using Avidin nano-carriers suppresses cytokine-induced catabolism long term [J].