Evaluation of gastrointestinal bacterial population for the production of holocellulose enzymes for biomass deconstruction

被引：20

作者：

Asem, Dhaneshwaree ^{[1
]}

Leo, Vincent Vineeth ^{[1
]}

Passari, Ajit Kumar ^{[1
]}

Tonsing, Mary Vanlalhruaii ^{[1
]}

Joshi, J. Beslin ^{[2
]}

Uthandi, Sivakumar ^{[2
]}

Hashem, Abeer ^{[3
]}

Abd Allah, Elsayed Fathi ^{[4
]}

Singh, Bhim Pratap ^{[1
]}

机构：

[1] Mizoram Univ, Dept Biotechnol, Mol Microbiol & Systemat Lab, Aizawl, Mizoram, India

[2] Tamil Nadu Agr Univ, Dept Agr Microbiol, Bioctalysts Lab, Coimbatore, Tamil Nadu, India

[3] King Saud Univ, Coll Sci, Bot & Microbiol Dept, Riyadh, Saudi Arabia

[4] King Saud Univ, Coll Food & Agr Sci, Plant Prod Dept, Riyadh, Saudi Arabia

来源：

PLOS ONE | 2017年 / 12卷 / 10期

关键词：

CELLULOLYTIC BACTERIA; XYLANASE PRODUCTION; CELLULASE; PRETREATMENT; DIVERSITY; RUMEN; OPTIMIZATION; PURIFICATION; STRATEGIES; ETHANOL;

D O I：

10.1371/journal.pone.0186355

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The gastrointestinal (GI) habitat of ruminant and non-ruminant animals sustains a vast ensemble of microbes that are capable of utilizing lignocellulosic plant biomass. In this study, an indigenous swine (Zovawk) and a domesticated goat (Black Bengal) were investigated to isolate bacteria having plant biomass degrading enzymes. After screening and enzymatic quantification of eighty-one obtained bacterial isolates, Serratia rubidaea strain DBT4 and Aneurinibacillus aneurinilyticus strain DBT87 were revealed as the most potent strains, showing both cellulase and xylanase production. A biomass utilization study showed that submerged fermentation (SmF) of D2 (alkaline pretreated pulpy biomass) using strain DBT4 resulted in the most efficient biomass deconstruction with maximum xylanase (11.98 U/mL) and FPase (0.5 U/mL) activities (55 degrees C, pH 8). The present study demonstrated that bacterial strains residing in the gastrointestinal region of non-ruminant swine are a promising source for lignocellulose degrading microorganisms that could be used for biomass conversion.

引用

页数：17

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