Influence of pH, temperature and the cationic porphyrin TMPyP4 on the stability of the i-motif formed by the 5′-(C3TA2)4-3′ sequence of the human telomere

被引：49

作者：

Fernandez, Sergio ^{[1
]}

Eritja, Ramon ^{[2
]}

Avino, Anna ^{[2
]}

Jaumot, Joaquim ^{[1
]}

Gargallo, Raimundo ^{[1
]}

机构：

[1] Univ Barcelona, Solut Equilibria & Chemometr Grp, Associate Unit UB CSIC, Dept Analyt Chem, E-08028 Barcelona, Spain

[2] CIBER BBN Networking Ctr Bioengn Biomat & Nanomed, Biomed Res Inst, IQAC CSIC, E-08028 Barcelona, Spain

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2011年 / 49卷 / 04期

关键词：

i-Motif; Human telomere; Multivariate analysis; Loops; TMPyP4; binding; MULTIVARIATE CURVE RESOLUTION; CYTOSINE-RICH STRAND; G-QUADRUPLEX; DNA; FRAGMENT; EQUILIBRIA; UPSTREAM; PROTEIN; DUPLEX; REGION;

D O I：

10.1016/j.ijbiomac.2011.07.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of pH, temperature and the cationic porphyrin TMPyP4 on the conformational equilibria of the cytosine-rich strand of the human telomeric sequence 5'-(C(3)TA(2))(4)-3', as well as those of the sequence 5'-(C3TT2)(4)-3', was studied. The presence of adenine bases at the loops causes the formation of two different intramolecular i-motif structures with a pH-transition midpoint around 4.6, which stability is lower than the i-motif formed by the sequence 5'-(C3TT2)(4)-3'. The stoichiometries of the complexes formed by these i-motif structures with TMPyP4 are also influenced by the presence of adenine at the loops. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：729 / 736

页数：8

共 42 条

[1] HUMAN TELOMERIC C-STRAND TETRAPLEXES [J].