Influence of pH, temperature and the cationic porphyrin TMPyP4 on the stability of the i-motif formed by the 5′-(C3TA2)4-3′ sequence of the human telomere

被引:47
作者
Fernandez, Sergio [1 ]
Eritja, Ramon [2 ]
Avino, Anna [2 ]
Jaumot, Joaquim [1 ]
Gargallo, Raimundo [1 ]
机构
[1] Univ Barcelona, Solut Equilibria & Chemometr Grp, Associate Unit UB CSIC, Dept Analyt Chem, E-08028 Barcelona, Spain
[2] CIBER BBN Networking Ctr Bioengn Biomat & Nanomed, Biomed Res Inst, IQAC CSIC, E-08028 Barcelona, Spain
关键词
i-Motif; Human telomere; Multivariate analysis; Loops; TMPyP4; binding; MULTIVARIATE CURVE RESOLUTION; CYTOSINE-RICH STRAND; G-QUADRUPLEX; DNA; FRAGMENT; EQUILIBRIA; UPSTREAM; PROTEIN; DUPLEX; REGION;
D O I
10.1016/j.ijbiomac.2011.07.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The influence of pH, temperature and the cationic porphyrin TMPyP4 on the conformational equilibria of the cytosine-rich strand of the human telomeric sequence 5'-(C(3)TA(2))(4)-3', as well as those of the sequence 5'-(C3TT2)(4)-3', was studied. The presence of adenine bases at the loops causes the formation of two different intramolecular i-motif structures with a pH-transition midpoint around 4.6, which stability is lower than the i-motif formed by the sequence 5'-(C3TT2)(4)-3'. The stoichiometries of the complexes formed by these i-motif structures with TMPyP4 are also influenced by the presence of adenine at the loops. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:729 / 736
页数:8
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