cfa-miR-143 Promotes Apoptosis via the p53 Pathway in Canine Influenza Virus H3N2-Infected Cells

被引：13

作者：

Zhou, Pei ^{[1
,2
,3
]}

Tu, Liqing ^{[1
,2
,3
]}

Lin, Xi ^{[1
,2
,3
]}

Hao, Xiangqi ^{[1
,2
,3
]}

Zheng, Qingxu ^{[1
,2
,3
]}

Zeng, Weijie ^{[1
,2
,3
]}

Zhang, Xin ^{[1
,2
,3
]}

Zheng, Yun ^{[1
,2
,3
]}

Wang, Lifang ^{[1
,2
,3
]}

Li, Shoujun ^{[1
,2
,3
]}

机构：

[1] South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

[2] Guangdong Prov Key Lab Prevent & Control Severe C, Guangzhou 510642, Guangdong, Peoples R China

[3] Guangdong Prov Pet Engn Technol Res Ctr, Guangzhou 510642, Guangdong, Peoples R China

来源：

VIRUSES-BASEL | 2017年 / 9卷 / 12期

基金：

中国国家自然科学基金;

关键词：

cfa-miR-143; canine influenza virus; apoptosis; p53; caspase; FACTOR-BINDING PROTEIN-5; A-VIRUS; MICRORNA EXPRESSION; RNA INTERFERENCE; CANCER CELLS; LUNG-TISSUE; GROWTH; ACTIVATION; IDENTIFICATION; PROLIFERATION;

D O I：

10.3390/v9120360

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

MicroRNAs regulate multiple aspects of the host response to viral infection. This study verified that the expression of cfa-miR-143 was upregulated in vivo and in vitro by canine influenza virus (CIV) H3N2 infection. To understand the role of cfa-miR-143 in CIV-infected cells, the target gene of cfa-miR-143 was identified and assessed for correlations with proteins involved in the apoptosis pathway. A dual luciferase reporter assay showed that cfa-miR-143 targets insulin-like growth factor binding protein 5 (Igfbp5). Furthermore, a miRNA agomir and antagomir of cfa-miR-143 caused the downregulation and upregulation of Igfbp5, respectively, in CIV-infected madin-darby canine kidney (MDCK) cells. This study demonstrated that cfa-miR-143 stimulated p53 and caspase3 activation and induced apoptosis via the p53 pathway in CIV H3N2-infected cells. In conclusion, CIV H3N2 induced the upregulation of cfa-miR-143, which contributes to apoptosis via indirectly activating the p53-caspase3 pathway.

引用

页数：14

共 62 条

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