Preparation of Electrochemical Sensor Based on Magnetic Graphene Nanocomposite for Determination of Dopamine

This work was focused on the preparation of an electrochemical sensor based on magnetic graphene nanocomposite for the determination of dopamine (DA) in human plasma samples. The electrodeposition method was used for the synthesis of magnetic graphene nanocomposite based on Fe-Ni bimetal oxides (Fe2O3-NiO) and graphene oxide (GO) on a glassy carbon electrode (GCE). Fe2O3-NiO@GO/GCE using XRD and SEM confirmed the simultaneous electrodeposition of Fe-Ni bimetal oxide nanoparticles in the spherical-shaped 2D wrinkled stack of ultra-thin GO nanosheets without any aggregation. Electrochemical analyses using DPV and amperometry techniques showed the good electrocatalytic activity, stability, and selectivity of Fe2O3-NiO@GO/GCE towards the oxidation of DA with rapid electron transfer and mass transport. Furthermore, the sensitivity and detection limit of magnetic graphene nanocomposite sensors were obtained at 0.16812 mu A/mu M and 0.005 mu M, respectively, which were compared with the other reported DA electrochemical sensors and the results indicated comparable detection limit values and broader linear range of Fe2O3NiO@GO/GCE for the determination of DA. The practical capability of Fe2O3-NiO@GO/GCE for the determination of DA was investigated in prepared real samples of human blood serum of six patients aged 55 to 70 years who were administered an intropin injection. Results showed that there was good agreement between the amperometry and ELISA measurements, and the obtained RSD ranged from 3.08% to 4.36% illustrated the acceptable accuracy of both techniques, especially Fe2O3NiO@GO/GCE as a reliable and accurate electrochemical DA sensor in clinical and pharmaceutical analyses.