Synthesis and properties of oligonucleotides containing 8-bromo-2′-deoxyguanosine

被引：13

作者：

Fàbrega, C ^{[1
]}

Macías, MJ ^{[1
]}

Eritja, R ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 03期

关键词：

D O I：

10.1081/NCN-100002085

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation of oligonucleotides containing 8-bromo-2 ' -deoxyguanosine is described. Substitution of G by 8-bromoguanine on an alternating CG decamer stabilizes the Z-form in such a way that the B-form was not observed. Melting temperatures showed that duplexes in which 8-bromo-2 ' -deoxyguanosine paired with natural bases were much less stable.

引用

收藏

页码：251 / 260

页数：10

相关论文

共 28 条

[1] THE SYNTHESIS OF MODIFIED OLIGONUCLEOTIDES BY THE PHOSPHORAMIDITE APPROACH AND THEIR APPLICATIONS [J].

BEAUCAGE, SL ;

IYER, RP .

TETRAHEDRON, 1993, 49 (28) :6123-6194

[2]

BHATNAGAR SK, 1991, J BIOL CHEM, V266, P9050

[3]

CONNOLLY BA, 1992, METHOD ENZYMOL, V211, P36

[4] CHEMICAL PROBE FOR GLYCOSIDIC CONFORMATION IN TELOMERIC DNAS [J].

DIAS, E ;

BATTISTE, JL ;

WILLIAMSON, JR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (10) :4479-4480

[5] Synthesis and characterization of 8-methoxy-2'-deoxyadenosine-containing oligonucleotides to probe the syn glycosidic conformation of 2'-deoxyadenosine within DNA [J].

Eason, RG ;

Burkhardt, DM ;

Phillips, SJ ;

Smith, DP ;

David, SS .

NUCLEIC ACIDS RESEARCH, 1996, 24 (05) :890-897

[6]

Efrati E, 1997, J BIOL CHEM, V272, P2559

[7] Studies on the synthesis of oligonucleotides containing photoreactive nucleosides:: 2-azido-2′-deoxyinosine and 8-azido-2′-deoxyadenosine [J].

Fàbrega, C ;

García, RG ;

Díaz, AR ;

Eritja, R .

BIOLOGICAL CHEMISTRY, 1998, 379 (4-5) :527-533

[8] Synthesis of oligodeoxynucleotides containing 5-aminouracil and its N-acetyl derivative [J].

Ferrer, E ;

Neubauer, G ;

Mann, M ;

Eritja, R .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1997, (14) :2051-2057

[9] Preparation and properties of oligodeoxynucleotides containing 5-iodouracil and 5-bromo- and 5-iodocytosine [J].

Ferrer, E ;

Wiersma, M ;

Kazimierczak, B ;

Muller, CW ;

Eritja, R .

BIOCONJUGATE CHEMISTRY, 1997, 8 (05) :757-761

[10] A RAPID METHOD FOR THE FUNCTIONALIZATION OF POLYMER SUPPORTS FOR SOLID-PHASE OLIGONUCLEOTIDE SYNTHESIS [J].

GUPTA, KC ;

KUMAR, P ;

BHATIA, D ;

SHARMA, AK .

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 1995, 14 (3-5) :829-832