Bovine coronavirus in the lower respiratory tract of cattle with respiratory disease

被引：21

作者：

Rahe, Michael C. ^{[1
]}

Magstadt, Drew R. ^{[1
]}

Groeltz-Thrush, Jennifer ^{[1
]}

Gauger, Phillip C. ^{[1
]}

Zhang, Jianqiang ^{[1
]}

Schwartz, Kent J. ^{[1
]}

Siepker, Christopher L. ^{[1
]}

机构：

[1] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, 1800 Christensen Dr, Ames, IA 50011 USA

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2022年 / 34卷 / 03期

关键词：

bovine coronavirus; histopathology; immunohistochemistry; respiratory disease; RNAscope; SYNCYTIAL VIRUS; CALVES; INFECTION;

D O I：

10.1177/10406387221078583

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bovine coronavirus (BCoV) is a known cause of enteric disease in cattle; however, its role in bovine respiratory disease (BRD) is poorly understood, with a dearth of evidence of the detection of the virus in respiratory tract lesions. We coupled histologic evaluation of tracheal and lower airway tissues from 104 calves with BRD in which BCoV was detected in the lungs via PCR followed by direct detection of BCoV by immunohistochemistry and an RNA in situ hybridization assay (ISH; RNAscope technology). RNAscope ISH detected BCoV in respiratory epithelium in more cases than did IHC. Using both methods of direct detection, tracheal epithelial attenuation and identification of the virus within lesions were observed commonly. Our results confirm a role of BCoV in respiratory tract infection and pathology, and show that the virus likely plays a role in the development of BRD.

引用

页码：482 / 488

页数：7

共 19 条

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