Current tools to interrogate microglial biology

被引：35

作者：

Dumas, Anaelle Aurelie ^{[1
]}

Borst, Katharina ^{[1
]}

Prinz, Marco ^{[1
,2
,3
,4
]}

机构：

[1] Univ Freiburg, Med Fac, Inst Neuropathol, Freiburg, Germany

[2] Univ Freiburg, Ctr NeuroModulat NeuroModBasics, Freiburg, Germany

[3] Univ Freiburg, Signalling Res Ctr BIOSS, Freiburg, Germany

[4] Univ Freiburg, Signalling Res Ctr CIBSS, Freiburg, Germany

来源：

NEURON | 2021年 / 109卷 / 18期

关键词：

PLURIPOTENT STEM-CELLS; CENTRAL-NERVOUS-SYSTEM; FRACTALKINE RECEPTOR CX(3)CR1; SINGLE-CELL; GENE-EXPRESSION; ALZHEIMERS-DISEASE; MYELOID CELLS; SPATIAL TRANSCRIPTOMICS; T-CELLS; REVEALS;

D O I：

10.1016/j.neuron.2021.07.004

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Microglial cells perform a plethora of functions in the central nervous system (CNS), involving them in brain development, maintenance of homeostasis in adulthood, and CNS diseases. Significant technical advancements have prompted the development of novel systems adapted to analyze microglia with increasing specificity and intricacy. The advent of single-cell technologies combined with targeted mouse models has been decisive in deciphering microglia heterogeneity and dissecting microglial functions. However sophisticated these tools have become, clear limitations remain. Understanding their pitfalls and advantages ensures their correct application. Therefore, we provide a guide to the cutting-edge methods currently available to dissect microglial biology.

引用

页码：2805 / 2819

页数：15

共 170 条

[1] iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases [J].

Abud, Edsel M. ;

Ramirez, Ricardo N. ;

Martinez, Eric S. ;

Healy, Luke M. ;

Nguyen, Cecilia H. H. ;

Newman, Sean A. ;

Yeromin, Andriy V. ;

Scarfone, Vanessa M. ;

Marsh, Samuel E. ;

Fimbres, Cristhian ;

Caraway, Chad A. ;

Fote, Gianna M. ;

Madany, Abdullah M. ;

Agrawal, Anshu ;

Kayed, Rakez ;

Gylys, Karen H. ;

Cahalan, Michael D. ;

Cummings, Brian J. ;

Antel, Jack P. ;

Mortazavi, Ali ;

Carson, Monica J. ;

Poon, Wayne W. ;

Blurton-Jones, Mathew .

NEURON, 2017, 94 (02) :278-+

[2] Single-cell mass cytometry reveals distinct populations of brain myeloid cells in mouse neuroinflammation and neurodegeneration models [J].

Ajami, Bahareh ;

Samusik, Nikolay ;

Wieghofer, Peter ;

Ho, Peggy P. ;

Crotti, Andrea ;

Bjornson, Zach ;

Prinz, Marco ;

Fantl, Wendy J. ;

Nolan, Garry P. ;

Steinman, Lawrence .

NATURE NEUROSCIENCE, 2018, 21 (04) :541-+

[3] Infiltrating monocytes trigger EAE progression, but do not contribute to the resident microglia pool [J].

Ajami, Bahareh ;

Bennett, Jami L. ;

Krieger, Charles ;

McNagny, Kelly M. ;

Rossi, Fabio M. V. .

NATURE NEUROSCIENCE, 2011, 14 (09) :1142-U263

[4] Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo [J].

Alvarez-Castelao, Beatriz ;

Schanzenbaecher, Christoph T. ;

Langer, Julian D. ;

Schuman, Erin M. .

NATURE PROTOCOLS, 2019, 14 (02) :556-575

[5] Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization [J].

Antunes, Ana Rita Pombo ;

Scheyltjens, Isabelle ;

Lodi, Francesca ;

Messiaen, Julie ;

Antoranz, Asier ;

Duerinck, Johnny ;

Kancheva, Daliya ;

Martens, Liesbet ;

De Vlaminck, Karen ;

Van Hove, Hannah ;

Hansen, Signe Schmidt Kjolner ;

Bosisio, Francesca Maria ;

Van der Borght, Koen ;

De Vleeschouwer, Steven ;

Sciot, Raf ;

Bouwens, Luc ;

Verfaillie, Michiel ;

Vandamme, Niels ;

Vandenbroucke, Roosmarijn E. ;

De Wever, Olivier ;

Saeys, Yvan ;

Guilliams, Martin ;

Gysemans, Conny ;

Neyns, Bart ;

De Smet, Frederik ;

Lambrechts, Diether ;

Van Ginderachter, Jo A. ;

Movahedi, Kiavash .

NATURE NEUROSCIENCE, 2021, 24 (04) :595-610

[6] Coupled Proliferation and Apoptosis Maintain the Rapid Turnover of Microglia in the Adult Brain [J].

Askew, Katharine ;

Li, Kaizhen ;

Olmos-Alonso, Adrian ;

Garcia-Moreno, Fernando ;

Liang, Yajie ;

Richardson, Philippa ;

Tipton, Tom ;

Chapman, Mark A. ;

Riecken, Kristoffer ;

Beccari, Sol ;

Sierra, Amanda ;

Molnar, Zoltan ;

Cragg, Mark S. ;

Garaschuk, Olga ;

Perry, V. Hugh ;

Gomez-Nicola, Diego .

CELL REPORTS, 2017, 18 (02) :391-405

[7] Generation of pure monocultures of human microglia-like cells from induced pluripotent stem cells [J].

Banerjee, Poulomi ;

Paza, Evdokia ;

Perkins, Emma M. ;

James, Owen G. ;

Kenkhuis, Boyd ;

Lloyd, Amy F. ;

Burr, Karen ;

Story, David ;

Yusuf, Dilmurat ;

He, Xin ;

Backofen, Rolf ;

Dando, Owen ;

Chandran, Siddharthan ;

Priller, Josef .

STEM CELL RESEARCH, 2020, 49

[8] Organoids in immunological research [J].

Bar-Ephraim, Yotam E. ;

Kretzschmar, Kai ;

Clevers, Hans .

NATURE REVIEWS IMMUNOLOGY, 2020, 20 (05) :279-293

[9] Wild-type microglia extend survival in PU.1 knockout mice with familial amyotrophic lateral sclerosis [J].

Beers, David R. ;

Henkel, Jenny S. ;

Xiao, Qin ;

Zhao, Weihua ;

Wang, Jinghong ;

Yen, Albert A. ;

Siklos, Laszlo ;

McKercher, Scott R. ;

Appel, Stanley H. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (43) :16021-16026

[10] A Combination of Ontogeny and CNS Environment Establishes Microglial Identity [J].

Bennett, F. Chris ;

Bennett, Mariko L. ;

Yaqoob, Fazeela ;

Mulinyawe, Sara B. ;

Grant, Gerald A. ;

Gephart, Melanie Hayden ;

Plowey, Edward D. ;

Barres, Ben A. .

NEURON, 2018, 98 (06) :1170-+

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