Correlative light and electron microscopy methods for the study of virus-cell interactions

被引：61

作者：

Bykov, Yury S. ^{[1
]}

Cortese, Mirko ^{[2
]}

Briggs, John A. G. ^{[1
]}

Bartenschlager, Ralf ^{[2
]}

机构：

[1] European Mol Biol Lab, Struct & Computat Biol Unit, D-69117 Heidelberg, Germany

[2] Heidelberg Univ, Dept Infect Dis, Mol Virol, Neuenheimer Feld 345, D-69120 Heidelberg, Germany

来源：

FEBS LETTERS | 2016年 / 590卷 / 13期

关键词：

correlative light and electron microscopy; electron microscopy; virus-host interaction; HEPATITIS-C VIRUS; SEMLIKI-FOREST-VIRUS; STANDARD FLUORESCENT PROTEINS; WEST-NILE-VIRUS; CRYOELECTRON TOMOGRAPHY; VITREOUS SECTIONS; SUPERRESOLUTION FLUORESCENCE; LOCALIZATION MICROSCOPY; MULTICELLULAR ORGANISMS; MEMBRANOUS REPLICATION;

D O I：

10.1002/1873-3468.12153

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron microscopy (EM) is an invaluable tool to study the interactions of viruses with cells, and the ultrastructural changes induced in host cells by virus infection. Light microscopy (LM) is a complementary tool with the potential to locate rare events, label specific components, and obtain dynamic information. The combination of LM and EM in correlative light and electron microscopy (CLEM) is particularly powerful. It can be used to complement a static EM image with dynamic data from live imaging, identify the ultrastructure observed in LM, or, conversely, provide molecular specificity data for a known ultrastructure. Here, we describe methods and strategies for CLEM, discuss their advantages and limitations, and review applications of CLEM to study virus-host interactions.

引用

页码：1877 / 1895

页数：19

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