Matrix Sublimation Device for MALDI Mass Spectrometry Imaging

被引：28

作者：

Fernandez, Roberto ^{[1
]}

Garate, Jone ^{[1
]}

Martin-Saiz, Lucia ^{[1
]}

Galetich, Igor ^{[1
]}

Fernandez, Jose A. ^{[1
]}

机构：

[1] Univ Basque Country UPV EHU, Fac Sci & Technol, Dept Phys Chem, Barrio Sarriena S-N, Leioa 48940, Spain

来源：

ANALYTICAL CHEMISTRY | 2019年 / 91卷 / 01期

关键词：

DEPOSITION; TISSUE; 2-MERCAPTOBENZOTHIAZOLE; ADVANCEMENTS; PROTEINS; LIPIDS; BRAIN;

D O I：

10.1021/acs.analchem.8b04765

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Sublimation is a widely used method for matrix deposition in imaging mass spectrometry experiments. Still, most of the time, standard glass sublimators are used for this purpose, which do not enable optimal matrix deposition reproducibility, compromising inter-experiment comparison of the results. Here, we present an in-house designed stainless steel sublimator in which the parameters that have the strongest influence over matrix deposition reproducibility can be easily monitored. Using sections of human colon biopsies, we demonstrate the capabilities of this new prototype.

引用

页码：803 / 807

页数：5

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