Kinetics and microbial community analysis for hydrogen production using raw grass inoculated with different pretreated mixed culture

被引：66

作者：

Yang, Guang ^{[1
]}

Wang, Jianlong ^{[1
,2
]}

机构：

[1] Tsinghua Univ, INET, Collaborat Innovat Ctr Adv Nucl Energy Technol, Beijing 100084, Peoples R China

[2] Tsinghua Univ, Beijing Key Lab Radioact Wastes Treatment, Beijing 100084, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2018年 / 247卷

基金：

中国国家自然科学基金;

关键词：

Inoculum pretreatment; Fermentative hydrogen production; Grass; Kinetic model; Microbial community; WASTE ACTIVATED-SLUDGE; BIOHYDROGEN PRODUCTION; SEWAGE-SLUDGE; METHANE PRODUCTION; CO-DIGESTION; FOOD WASTE; FERMENTATION; BACTERIA; PERFORMANCE; RYEGRASS;

D O I：

10.1016/j.biortech.2017.09.041

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In this study, five pretreatment methods (heat shock, acid, base, aeration and gamma radiation) were applied for enriching hydrogen producers from anaerobically digested sludge, aiming to compare their hydrogen fermentation performance using raw ryegrass as substrate. Results showed that various pretreatment methods caused great variations on grass hydrogen fermentation performance. Acid pretreatment was most efficient compared with other tested pretreatment methods, with relevant hydrogen yield of 64.4 mL/g dry grass and organics removal of 31.4%. Kinetics results showed that the first-order kinetic model fitted hydrogen evolution better than the modified Gompertz model. Microbiological analysis showed that various pretreatment methods caused great variations on microbial activity and microbial community composition. Clostridium and Enterococcus were two dominant genera, while relative abundances of these two genera varied greatly for different pretreated samples. Difference in microbial activity and microbial community distribution induced by the pretreatment methods might directly cause different ryegrass fermentation performance.

引用

页码：954 / 962

页数：9

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