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A quaternary ammonium modified coumarin derivative for antimicrobial photodynamic therapy
被引:0
作者:
Sun, Zhiyuan
[1
,3
]
Zhou, Shaona
[2
]
Gu, Ying
[2
]
Zhao, Yuxia
[1
]
机构:
[1] Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100190, Peoples R China
[2] Chinese Peoples Liberat Army Gen Hosp, Dept Laser Med, Beijing 100049, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源:
LIGHT-BASED DIAGNOSIS AND TREATMENT OF INFECTIOUS DISEASES
|
2018年
/
10479卷
基金:
中国国家自然科学基金;
关键词:
photodynamic therapy;
antibacterial;
antifungal;
coumarin derivative;
quaternary ammonium;
PHOTOSENSITIZERS;
SERIES;
OXYGEN;
D O I:
10.1117/12.2287086
中图分类号:
R51 [传染病];
学科分类号:
100401 ;
摘要:
A new cationic modified coumarin derivative, 7-diethylamino-3-(3-(4-(trimethylbenzenaminium iodide) phenyl) acryloyl)-2H-chromen-2-one (1), was synthesized and characterized by H-1 NMR and mass spectra. It had a strong intramolecular charge transfer absorption band around 460 nm with large molar extinction coefficients of 3.94 x 10(4) M-1 cm(-1) in DMF and 3.86 x 10(4) M-1 cm(-1) in PBS, respectively. Moreover, a moderate singlet oxygen quantum yield of 0.16 was obtained for 1 in DMF. Using methylene blue (MB) under a 630 nm laser as reference, the in vitro antimicrobial photodynamic therapy (aPDT) activity of 1 against three strains, gram positive bacteria methicillin-resistant staphylococcus aureus (MRSA), negative bacteria acinetobacter baumannii (A. baumannii) and fungus Candida albicans (C. albicans), was carried out by irradiation with a 457 nm laser. It was shown that 1 had no dark toxicity to these bacteria when its concentration was up to 100 mu M, while under the 457 nm laser it could kill them effectively with an over 3 log CFU/ml decrease of the bacterial viability with its concentration up to 5 mu M. The aPDT capability of 1 against MRSA and A. baumannii was equivalent to that of MB. For C. albicans, 1 exhibited much better aPDT effect than MB.
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页数:8
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