Human-like smelling of a rose scent using an olfactory receptor nanodisc-based bioelectronic nose

被引：40

作者：

Lee, Minju ^{[1
,2
]}

Yang, Heehong ^{[3
,4
]}

Kim, Daesan ^{[5
]}

Yang, Myungjae ^{[1
,2
]}

Park, Tai Hyun ^{[3
]}

Hong, Seunghun ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Dept Phys & Astron, Seoul 08826, South Korea

[2] Seoul Natl Univ, Inst Appl Phys, Seoul 08826, South Korea

[3] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 08826, South Korea

[4] MOGAM Inst Biomed Res, Recombinants Unit, Prot Engn Lab, Yongin 16924, South Korea

[5] Seoul Natl Univ, Dept Biophys & Chem Biol, Seoul 08826, South Korea

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

欧洲研究理事会; 新加坡国家研究基金会;

关键词：

ODORANT RECEPTORS; ESSENTIAL OIL; TRANSISTOR; PROTEIN; LIGAND; DISCRIMINATION; GERANIOL; PLATFORM; BINDING; SYSTEM;

D O I：

10.1038/s41598-018-32155-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report a strategy for the human-like smelling of a rose scent utilizing olfactory receptor nanodisc (ND)-based bioelectronic nose devices. In this strategy, a floating electrode (FE)-based carbon nanotube (CNT) field effect transistor (FET) was functionalized with human olfactory receptor 1A2 (hOR1A2)embedded NDs (hOR1A2NDs). The hOR1A2NDs responded to rose scent molecules specifically, which were monitored electrically using the underlying CNT-FET. This strategy allowed us to quantitatively assess the contents of geraniol and citronellol, the main components of a rose scent, as low as 1 fM and 10 fM, respectively. In addition, it enabled us to selectively discriminate a specific rose odorant from other odorants. Significantly, we also demonstrated that the responses of hOR1A2NDs to a rose scent could be strongly enhanced by enhancer materials like a human nose. Furthermore, the method provided a means to quantitatively evaluate rose scent components in real samples such as rose oil. Since our method allows one to quantitatively evaluate general rose scent ingredients just like a human nose, it could be a powerful strategy for versatile basic research and various applications such as fragrance development.

引用

页数：12

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