Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae

被引：93

作者：

Dai, Zhongxue ^{[1
]}

Gu, Honglian ^{[1
]}

Zhang, Shangjie ^{[1
]}

Xin, Fengxue ^{[1
]}

Zhang, Wenming ^{[1
,2
]}

Dong, Weiliang ^{[1
]}

Ma, Jiangfeng ^{[1
]}

Jia, Honghua ^{[1
,2
]}

Jiang, Min ^{[1
,2
]}

机构：

[1] Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Puzhu South Rd 30, Nanjing 211800, Jiangsu, Peoples R China

[2] Nanjing Tech Univ, Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 211800, Jiangsu, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 245卷

基金：

中国博士后科学基金;

关键词：

Methanol; Non-methylotrophy; S; cerevisiae; Synthetic biology; Pyruvate; BACILLUS-METHANOLICUS; BIOLOGICAL CONVERSION; HANSENULA-POLYMORPHA; ESCHERICHIA-COLI; CODON USAGE; ACID; EXPRESSION;

D O I：

10.1016/j.biortech.2017.05.100

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The aim of this study was to metabolically construct Saccharomyces cerevisiae for achievement of direct methanol utilization and value added product (mainly pyruvate) production. After successful integration of methanol oxidation pathway originated from Pichia pastoris into the chromosome of S. cerevisiae, the recombinant showed 1.04 g/L consumption of methanol and 3.13% increase of cell growth (OD600) when using methanol as the sole carbon source. Moreover, 0.26 g/L of pyruvate was detected in the fermentation broth. The supplementation of 1 g/L yeast extract could further improve cell growth with increase of 11.70% and methanol consumption to 2.35 g/L. This represents the first genetically modified nonmethylotrophic eukaryotic microbe for direct methanol utilization and would be of great value concerning the development of biotechnological processes. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：1407 / 1412

页数：6

共 24 条

[1] Engineering and analysis of a Saccharomyces cerevisiae strain that uses formaldehyde as an auxiliary substrate [J].