Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli

被引：1
作者：
Kitamatsu, Mizuki [1 ]
Kurami, Shunsuke [1 ]
Ohtsuki, Takashi [1 ]
Sisido, Masahiko [1 ]
机构：
[1] Okayama Univ, Grad Sch Nat Sci & Technol, Dept Med & Bioengn, Kita Ku, Okayama 7000082, Japan
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2011年 / 21卷 / 01期
关键词：
Peptide nucleic acid; Cell-penetrating peptide; Antisense effect; PNA; DNA; HYBRIDIZATION; INHIBITION; GROWTH; CELLS; RNA;
D O I：
10.1016/j.bmcl.2010.11.034
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-L-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：225 / 227
页数：3
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