Two isomeric and distinguishable H2S fluorescence probes for monitoring spoilage of eggs and visualizing exogenous and endogenous H2S in living cells

被引:0
作者
He, Yuqian [1 ]
Zhao, Bing [1 ]
Kan, Wei [1 ]
Ding, Limin [2 ]
Yu, Zhaochuan [1 ]
Wang, Mingyue [1 ]
Song, Bo [1 ]
Wang, Liyan [1 ]
机构
[1] Qiqihar Univ, Chem & Chem Engn Inst, Qiqihar 161006, Peoples R China
[2] First Hosp Qiqihar City, Cadre Ward, Qiqihar 161005, Peoples R China
基金
中国国家自然科学基金;
关键词
HYDROGEN-SULFIDE; SULFUR-COMPOUNDS; IN-VIVO; GAS; MITOCHONDRIA; METABOLISM; GENERATION; THIOLYSIS; SENSORS; FE3+;
D O I
10.1039/c9an01629e
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Accurate fabrication of fluorescence probes to efficiently monitor and detect H2S levels in the fields of foodstuffs and physiology is crucial. Herein, we report two isomeric phenanthro[9,10-d]imidazole benzene sulfonamide-derived fluorescence probes (PI-2-SA and PI-4-SA), both of which display remarkable responses toward H2S over other analytes. The spectral characteristics of the two probes were investigated and are discussed in detail. By comparison, PI-2-SA was specific, sensitive (the limit of detection was ca. 12.3 nM), rapid (within <= 3 min) and dynamic (the rate constant was 0.02 s(-1)). Significantly, PI-2-SA was proved to be effective in monitoring the shelf-time progress of egg samples in real time as well as in the imaging of exogenous and endogenous H2S in HeLa cells.
引用
收藏
页码:213 / 222
页数:10
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