Characterization of the Largest Secretory Protein Family, Ricin B Lectin-like Protein, in Nosema bombycis: Insights into Microsporidian Adaptation to Host

被引:10
作者
Xu, Jinzhi [1 ,2 ]
Luo, Jian [1 ,2 ]
Chen, Jiajing [1 ,2 ]
Vossbrinck, Charles R. [3 ]
Li, Tian [1 ,2 ]
Zhou, Zeyang [1 ,2 ,4 ]
机构
[1] Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China
[2] Southwest Univ, Chongqing Key Lab Microsporidia Infect & Control, Chongqing 400715, Peoples R China
[3] Connecticut Agr Expt Stn, Dept Environm Sci, 123 Huntington St, New Haven, CT 06511 USA
[4] Chongqing Normal Univ, Coll Life Sci, Chongqing 400047, Peoples R China
基金
中国国家自然科学基金;
关键词
microsporidia; Nosema bombycis; Ricin B lectin-like; secreted protein; evolution; HOMOLOGOUS RECOMBINATION; TRANSCRIPTION; VIRULENCE; REVEALS; TARGETS; CHAIN;
D O I
10.3390/jof8060551
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Microsporidia are a group of obligate intracellular pathogens infecting nearly all animal phyla. The microsporidian Nosema bombycis has been isolated from several lepidopteran species, including the economy-important silkworms as well as several crop pests. Proteins secreted by parasites can be important virulent factors in modulating host pathways. Ricin is a two-chain lectin best known for its extreme vertebrate toxicity. Ricin B lectin-like proteins are widely distributed in microsporidia, especially in N. bombycis. In this study, we identify 52 Ricin B lectin-like proteins (RBLs) in N. bombycis. We show that the N. bombycis RBLs (NbRBLs) are classified into four subfamilies. The subfamily 1 was the most conserved, with all members having a Ricin B lectin domain and most members containing a signal peptide. The other three subfamilies were less conserved, and even lost the Ricin B lectin domain, suggesting that NbRBLs might be a multi-functional family. Our study here indicated that the NbRBL family had evolved by producing tandem duplications firstly and then expanded by segmental duplications, resulting in concentrated localizations mainly in three genomic regions. Moreover, based on RNA-seq data, we found that several Nbrbls were highly expressed during infection. Further, the results show that the NbRBL28 was secreted into host nucleus, where it promotes the expressions of genes involved in cell cycle progression. In summary, the great copy number, high divergence, and concentrated genome distribution of the NbRBLs demonstrated that these proteins might be adaptively evolved and played a vital role in the multi-host N. bombycis.
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页数:15
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