A Highly Selective and Sensitive Fluorescence Detection Method of Glyphosate Based on an Immune Reaction Strategy of Carbon Dot Labeled Antibody and Antigen Magnetic Beads

被引：108

作者：

Wang, Duo ^{[1
]}

Lin, Bixia ^{[1
]}

Cao, Yujuan ^{[1
]}

Guo, Manli ^{[1
]}

Yu, Ying ^{[1
,2
]}

机构：

[1] South China Normal Univ, Guangzhou Key Lab Analyt Chem Biomed, Sch Chem & Environm, Guangzhou 510006, Guangdong, Peoples R China

[2] 378 Waihuan West Rd, Guangzhou 510006, Guangdong, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2016年 / 64卷 / 30期

基金：

中国国家自然科学基金;

关键词：

glyphosate; carbon dots; magnetic beads; fluorescence immunoassay; quantitative determination; SOLID-PHASE EXTRACTION; LIQUID-CHROMATOGRAPHY; AMINOMETHYLPHOSPHONIC ACID; LEVEL DETERMINATION; GAS-CHROMATOGRAPHY; MASS-SPECTROMETRY; WATER SAMPLES; GLUFOSINATE; IMMUNOASSAY; CELLS;

D O I：

10.1021/acs.jafc.6b01088

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A sensitive fluorescence detection method for glyphosate (GLY) was established based on immune reaction. First, carbon dot labeled antibodies (IgG-CDs) which were able to specifically identify glyphosate were prepared with the environmentally friendly carbon dots (CDs) and glyphosate antibody (lgG). lgG-CDs could be used to in situ visualize the distribution of glyphosate in plant tissues. In order to eliminate the effects of excess lgG-CDs on the determination of GLY, antigen magnetic beads Fe3O4-GLY based on magnetic nanoparticles Fe3O4 and glyphosate were constructed and utilized to couple with the excess IgG-CDs. After magnetic separation to remove antigen magnetic beads, there was a linear relationship between the fluorescence intensity of lgG-CDs and the logarithmic concentration of glyphosate in the range of 0.01-80 mu g/mL with a detection limit of 8 ng/mL. The method was used for the detection of glyphosate in Pearl River water, tea, and soil samples with satisfactory recovery ratio between 87.4% and 103.7%.

引用

页码：6042 / 6050

页数：9

共 35 条

[1] Glyphosate Detection by Means of a Voltammetric Electronic Tongue and Discrimination of Potential Interferents [J].