Generation of homozygous PRKN, PINK1 and double PINK1/PRKN knockout cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing

被引：10

作者：

Chen, Carol X. -Q. ^{[1
]}

You, Zhipeng ^{[1
]}

Abdian, Narges ^{[1
]}

Sirois, Julien ^{[1
]}

Shlaifer, Irina ^{[1
]}

Tabatabaei, Mahdieh ^{[2
]}

Boivin, Marie-Noelle ^{[2
]}

Gaborieau, Lydiane ^{[2
]}

Karamchandani, Jason ^{[2
]}

Beitel, Lenore K. ^{[1
]}

Fon, Edward A. ^{[3
,4
]}

Durcan, Thomas M. ^{[1
]}

机构：

[1] McGill Univ, Neuros Early Drug Discovery Unit EDDU, 3801 Univ St, Montreal, PQ H3A 2B4, Canada

[2] McGill Univ, C BIG Repository C BIG, Montreal Neurol Inst, Montreal, PQ H3A 2B4, Canada

[3] Montreal Neurol Inst, Dept Neurol & Neurosurg, McGill Parkinson Program, Montreal, PQ H3A 2B4, Canada

[4] Montreal Neurol Inst, Dept Neurol & Neurosurg, Neurodegenerat Dis Grp, Montreal, PQ H3A 2B4, Canada

来源：

STEM CELL RESEARCH | 2022年 / 62卷

关键词：

D O I：

10.1016/j.scr.2022.102806

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Autosomal recessive mutations in either PRKN or PINK1 are associated with early-onset Parkinson's disease. The corresponding proteins, PRKN, an E3 ubiquitin ligase, and the mitochondrial serine/threonine-protein kinase PINK1 play a role in mitochondrial quality control. Using CRISPR/CAS9 technology we generated three human iPSC lines from the well characterized AIW002-02 control line. These isogenic iPSCs contain homozygous knockouts of PRKN (PRKN-KO, CBIGi001-A-1), PINK1 (PINK1-KO, CBIGi001-A-2) or both PINK1 and PRKN (PINK1-KO/PRKN-KO, CBIGi001-A-3). The knockout lines display normal karyotypes, express pluripotency markers and upon differentiation into relevant brain cells or midbrain organoids may be valuable tools to model Parkinson's disease.

引用

页数：7

共 50 条

[31] Generation of FOS gene knockout lines from a human embryonic stem cell line using CRISPR/Cas9 [J].

Li, Chunfu ;

Wang, Qing ;

Peng, Zhiyong ;

Lin, Yuchen ;

Liu, Huaying ;

Yang, Xiuling ;

Li, Shan ;

Liu, XiaoTing ;

Chen, Jingru .

STEM CELL RESEARCH, 2019, 39

[32] Derivation of Induced Pluripotent Stem Cells Line (RCPCMi009-A-1) with Knockout of the UBE2A Gene Using CRISPR/Cas9 Genome Editing [J].

E. A. Khomyakova ;

A. V. Fedorenko ;

A. V. Surdina ;

E. A. Volovikov ;

L. D. Belikova ;

E. A. Zerkalenkova ;

M. A. Lagarkova ;

A. N. Bogomazova .

Russian Journal of Developmental Biology, 2023, 54 :365-373

[33] Derivation of Induced Pluripotent Stem Cells Line (RCPCMi009-A-1) with Knockout of the UBE2A Gene Using CRISPR/Cas9 Genome Editing [J].

Khomyakova, E. A. ;

Fedorenko, A. V. ;

Surdina, A. V. ;

Volovikov, E. A. ;

Belikova, L. D. ;

Zerkalenkova, E. A. ;

Lagarkova, M. A. ;

Bogomazova, A. N. .

RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY, 2023, 54 (06) :365-373

[34] Generation of a heterozygous and a homozygous CSF1R knockout line from iPSC using CRISPR/Cas9 [J].

Schmitz, Anne S. ;

Korneck, Milena ;

Raju, Janani ;

Lamsfus-Calle, Andres ;

Daniel-Moreno, Alberto ;

Antony, Justin S. ;

Mezger, Markus ;

Schoels, Ludger ;

Hauser, Stefan ;

Hayer, Stefanie N. .

STEM CELL RESEARCH, 2023, 69

[35] Generation of a TRPV1 knockout human pluripotent stem cell line (WAe009-A-U) using CRISPR/Cas9 [J].

Cao, Yuhong ;

Tang, Ling ;

Su, Jun ;

Wang, Hao ;

Liang, Ping ;

Rong, Kuang ;

Gong, Tingyu .

STEM CELL RESEARCH, 2023, 72

[36] Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing [J].

Wu, Feima ;

Gao, Ge ;

Pan, Tingcai ;

Yang, Zhen ;

Xu, Guosheng ;

Abbas, Nasir ;

Liu, Yanli ;

Chen, Yan ;

Tan, Shenglin ;

You, Kai ;

Ke, Xinrong ;

Zhuang, Yuanqi ;

Lin, Xianhua ;

Yang, Fan ;

Li, Yin-xiong .

STEM CELL RESEARCH, 2018, 27 :5-9

[37] Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9 [J].

Kim, So-Jung ;

Habib, Omer ;

Kim, Jin-Soo ;

Han, Hyo-Won ;

Koo, Soo Kyung ;

Kim, Jung-Hyun .

STEM CELL RESEARCH, 2017, 19 :46-48

[38] Generation of a WWTR1 mutation induced pluripotent stem cell line, MUSIi012-A-1, using CRISPR/Cas9 [J].

Lorthongpanich, Chanchao ;

Jiamvoraphong, Nittaya ;

Supakun, Prapasri ;

Damkham, Nattaya ;

Terbto, Papussorn ;

Waeteekul, Supaporn ;

U-Pratya, Yaowalak ;

Laowtammathron, Chuti ;

Issaragrisil, Surapol .

STEM CELL RESEARCH, 2019, 41

[39] Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing [J].

Tsukamoto, Satomi ;

Nakade, Koji ;

Wakabayashi, Tamami ;

Nakashima, Kenichi ;

Takami, Miho ;

Hemmi, Yasuko ;

Kuramochi, Yuji ;

Shimizu, Tomoya ;

Arai, Yutaka ;

Matsuo-Takasaki, Mami ;

Noguchi, Michiya ;

Nakamura, Yukio ;

Miwa, Yoshihiro ;

Hayashi, Yohei .

STEM CELL RESEARCH, 2021, 53

[40] CRISPR/Cas9 Genome Editing to Repair Receptor-Mediated Endocytosis in Homozygous Familial Hypercholesterolemia Induced Pluripotent Stem Cells [J].

Omer, Linda ;

Hudson, Elizabeth A. ;

Hoying, Jay B. ;

Boyd, Nolan L. .

FASEB JOURNAL, 2017, 31

← 1 2 3 4 5 →