Reversible Ratiometric Fluorescent Probe for Sensing Bisulfate/H2O2 and Its Application in Zebrafish

被引：148

作者：

Zhang, Weijie ^{[1
]}

Liu, Tao ^{[1
]}

Huo, Fangjun ^{[2
]}

Ning, Peng ^{[3
]}

Meng, Xiangming ^{[3
]}

Yin, Caixia ^{[1
]}

机构：

[1] Shanxi Univ, Key Lab Mat Energy Convers & Storage Shanxi Prov, Minist Educ, Key Lab Chem Biol & Mol Engn,Inst Mol Sci, Taiyuan 030006, Shanxi, Peoples R China

[2] Shanxi Univ, Res Inst Appl Chem, Taiyuan 030006, Shanxi, Peoples R China

[3] Anhui Univ, Dept Chem, Hefei 230601, Anhui, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 15期

基金：

中国国家自然科学基金;

关键词：

SULFUR-DIOXIDE DERIVATIVES; OXIDATIVE STRESS; SELECTIVE DETECTION; SO2; DERIVATIVES; LIVING CELLS; HYDROGEN-PEROXIDE; MITOCHONDRIA; BISULFITE; WATER; H2O2;

D O I：

10.1021/acs.analchem.7b01580

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein, a novel near-infrared fluorescent probe for ratiometric detection of bisulfate was designed and developed based on a conjugation of naphthopyran-benzothiazolium system. The sensor showed excellent selectivity, high sensitivity and a rapid response toward bisulfite in aqueous solution. Upon the addition of HSO3-, the sensor displayed 37-fold (I-520/I-630) fluorescence intensity enhancement, accompanied by an apparent color change from violet to colorless, suggesting that the sensor can be used to detect HSO3- with "naked-eye". Notably, the addition product can be applied to the design of regenerative chemodosimeters based on the H2O2 promoted elimination of bisulfite and recovery of probe 1. Further cell and zebrafish imaging experiment demonstrated that the sensor could image the bisulfite/H2O2 redox cycle in biological system with ratiometric manners.

引用

页码：8079 / 8083

页数：5

共 27 条

[1] Fluorescent indicators based on BODIPY [J].

Boens, Noel ;

Leen, Volker ;

Dehaen, Wim .

CHEMICAL SOCIETY REVIEWS, 2012, 41 (03) :1130-1172

[2] Mitochondrial free radical generation, oxidative stress, and aging [J].

Cadenas, E ;

Davies, KJA .

FREE RADICAL BIOLOGY AND MEDICINE, 2000, 29 (3-4) :222-230

[3] Novel Coumarin-based Fluorescent Probe for Selective Detection of Bisulfite Anion in Water [J].

Chen Kangyu ;

Guo Yuan ;

Lu Zhenhuan ;

Yang Bingqin ;

Shi Zhen .

CHINESE JOURNAL OF CHEMISTRY, 2010, 28 (01) :55-60

[4] Outdoor air pollution: Nitrogen dioxide, sulfur dioxide, and carbon monoxide health effects [J].

Chen, Tze-Ming ;

Gokhale, Janaki ;

Shofer, Scott ;

Kuschner, Ware G. .

AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 2007, 333 (04) :249-256

[5] Oxidants, oxidative stress and the biology of ageing [J].

Finkel, T ;

Holbrook, NJ .

NATURE, 2000, 408 (6809) :239-247

[6] Cellular defenses against superoxide and hydrogen peroxide [J].

Imlay, James A. .

ANNUAL REVIEW OF BIOCHEMISTRY, 2008, 77 :755-776

[7] "Donor-Two-Acceptor" Dye Design: A Distinct Gateway to NIR Fluorescence [J].

Karton-Lifshin, Naama ;

Albertazzi, Lorenzo ;

Bendikov, Michael ;

Baran, Phil S. ;

Shabat, Doron .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (50) :20412-20420

[8] The PerR transcription factor senses H2O2 by metal-catalysed histidine oxidation [J].

Lee, JW ;

Helmann, JD .

NATURE, 2006, 440 (7082) :363-367

[9] A mitochondria-targeted fluorescent probe for ratiometric detection of endogenous sulfur dioxide derivatives in cancer cells [J].

Li, Dong-Peng ;

Wang, Zhao-Yang ;

Cao, Xiang-Jian ;

Cui, Jie ;

Wang, Xin ;

Cui, Hao-Zhong ;

Miao, Jun-Ying ;

Zhao, Bao-Xiang .

CHEMICAL COMMUNICATIONS, 2016, 52 (13) :2760-2763

[10] A water-soluble and fast-response mitochondria-targeted fluorescent probe for colorimetric and ratiometric sensing of endogenously generated SO2 derivatives in living cells [J].

Liu, Yu ;

Li, Kun ;

Xie, Ke-Xin ;

Li, Ling-Ling ;

Yu, Kang-Kang ;

Wang, Xin ;

Yu, Xiao-Qi .

CHEMICAL COMMUNICATIONS, 2016, 52 (16) :3430-3433

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