Enhanced production of butanol and acetoin by heterologous expression of an acetolactate decarboxylase in Clostridium acetobutylicum

被引:11
作者
Shen, Xiaoning [1 ]
Liu, Dong [1 ]
Liu, Jun [1 ]
Wang, Yanyan [1 ]
Xu, Jiahui [1 ]
Yang, Zhengjiao [1 ]
Guo, Ting [1 ]
Niu, Huanqing [1 ]
Ying, Hanjie [1 ]
机构
[1] Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, 30 Puzhu South Rd, Nanjing 211816, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Acetoin; Butanol; Clostridium acetobutylicum; Acetolactate decarboxylase; Zinc supplementation; ETHANOL FERMENTATION; ESCHERICHIA-COLI; GENE;
D O I
10.1016/j.biortech.2016.05.121
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Butanol is an important industrial chemical and an attractive transportation fuel. However, the deficiency of reducing equivalents NAD(P)H in butanol fermentation results in a large quantity of oxidation products, which is a major problem limiting the atom economy and economic viability of bio-butanol processes. Here, we integrated the butanol fermentation process with a NADH-generating, acetoin biosynthesis process to improve the butanol production. By overexpressing the a-acetolactate decarboxylase gene alsD from Bacillus subtilis in Clostridium acetobutylicum, acetoin yield was significantly increased at the cost of acetone. After optimization of fermentation conditions, butanol ( 12.9 g/L), acetoin ( 6.5 g/L), and ethanol ( 1.9 g/L) were generated by the recombinant strain, with acetone no more than 1.8 g/L. Thus, both mass yield and product value were greatly improved. This study demonstrates that reducing power compensation is effective to improve the atom economy of butanol fermentation, and provides a novel approach to improve the economic viability of bio-butanol production. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:601 / 606
页数:6
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